標題: Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp
作者: Lai, Hung-Chiao
Ng, Tze Hann
Ando, Masahiro
Lee, Chung-Te
Chen, I-Tung
Chuang, Jie-Cheng
Mavichak, Rapeepat
Chang, Sheng-Hsiung
Yeh, Mi-De
Chiang, Yi-An
Takeyama, Haruko
Hamaguchi, Hiro-o
Lo, Chu-Fang
Aoki, Takashi
Wang, Han-Ching
理學院
College of Science
關鍵字: Acute hepatopancreatic necrosis disease (AHPND);Early mortality syndrome (EMS);Vibrio parahaemolyticus;Shrimp
公開日期: 1-Dec-2015
摘要: Acute hepatopancreatic necrosis disease (AHPND), also called early mortality syndrome (EMS), is a recently emergent shrimp bacterial disease that has resulted in substantial economic losses since 2009. AHPND is known to be caused by strains of Vibrio parahaemolyticus that contain a unique virulence plasmid, but the pathology of the disease is still unclear. In this study, we show that AHPND-causing strains of V. parahaemolyticus secrete the plasmid encoded binary toxin PirAB(vp) into the culture medium. We further determined that, after shrimp were challenged with AHPND-causing bacteria, the bacteria initially colonized the stomach, where they started to produce PirAB(vp) toxin. At the same early time point (6 hpi), PirB(vp) toxin, but not PirA(vp) toxin, was detected in the hepatopancreas, and the characteristic histopathological signs of AHPND, including sloughing of the epithelial cells of the hepatopancreatic tubules, were also seen. Although some previous studies have found that both components of the binary PirAB(vp) toxin are necessary to induce a toxic effect, our present results are consistent with other studies which have suggested that PirB(vp) alone may be sufficient to cause cellular damage. At later time points, the bacteria and PirA(vp) and PirB(vp) toxins were all detected in the hepatopancreas. We also show that Raman spectroscopy "Whole organism fingerprints" were unable to distinguish between AHPND-causing and non-AHPND causing strains. Lastly, by using minimum inhibitory concentrations, we found that both virulent and non-virulent V. parahaemolyticus strains were resistant to several antibiotics, suggesting that the use of antibiotics in shrimp culture should be more strictly regulated. (C) 2015 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.fsi.2015.11.008
http://hdl.handle.net/11536/147826
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2015.11.008
期刊: FISH & SHELLFISH IMMUNOLOGY
Volume: 47
起始頁: 1006
結束頁: 1014
Appears in Collections:Articles