標題: IMPACT OF BLOOD MIXING AND ABO COMPATIBILITY ON PLATELET-LEUKOCYTE AGGREGATIONS AND PLATELET P-SELECTIN EXPRESSION: AN IN VITRO STUDY
作者: Huang, Go-Shine
Hu, Mei-Hua
Lin, Tso-Chou
Tsai, Yi-Ting
Lin, Chih-Yuan
Ke, Hung-Yen
Zheng, Xu-Zhi
Lin, Yi-Chang
Tsai, Chien-Sung
生物科技學系
Department of Biological Science and Technology
關鍵字: Blood transfusion;blood types;CD11a;CD11b;CD18;platelet-leukocyte aggregations;platelets;P-selectin
公開日期: 1-May-2018
摘要: Effects of blood transfusions on platelet- and leukocyte-related inflammation are unclear. We simulated transfusion using in vitro blood mixing to evaluate platelet-leukocyte aggregations (PLA) and platelet P-selectin expression, and the mechanism of PLA. Donor packed red blood cells (pRBCs) were obtained from a blood bank. Recipient whole blood samples were obtained from patients undergoing cardiac surgery. Blood sample mixtures were divided into four groups: group M, cross-matched blood type mixing; group O, donor type O with other blood type mixing (A, B, or AB); group S, ABO type-specific uncross-matched blood mixing; and group I, ABO incompatibility mixing. Donor pRBCswere added to recipient blood to reach 1%, 5%, and 10% (vol/vol) concentrations. Blood sample mixtures were analyzed to determine the PLA; P-selectin expression; and leukocyte CD11a, CD11b, and CD18 subunits of integrin expression. Analysis of variance tests were used to analyze differences. PLA significantly increased only in groups O and I (P=0.003 and P<0.001). Subpopulations of leukocytes significantly increased in all groups. There were no significant differences among the four groups (P=0.578) in PLA increase. Although there was no significant effect on P-selectin expression (P=1.000) and leukocyte CD11a and CD18 expression (P=0.999, P=0.422) within and between the groups, there was an increase in CD11b expression (P=0.018). Blood mixing can increase PLA, especially in platelet-neutrophil and platelet-monocyte aggregations, possibly through nonhemolytic reactions. The CD11b integrin with CD18 may play a role in the formation of PLA.
URI: http://dx.doi.org/10.1097/SHK.0000000000000972
http://hdl.handle.net/11536/147990
ISSN: 1073-2322
DOI: 10.1097/SHK.0000000000000972
期刊: SHOCK
Volume: 49
起始頁: 514
結束頁: 521
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