標題: Ultrasensitive Electrical Detection of Follicle-Stimulating Hormone Using a Functionalized Silicon Nanowire Transistor Chemosensor
作者: Lee, Mucian
Palanisamy, Sathyadevi
Zhou, Bin-Hou
Wang, Li-Yu
Chen, Chiao-Yun
Lee, Chen-Yi
Yuan, Shyng-Shiou F.
Wang, Yun-Ming
交大名義發表
電子工程學系及電子研究所
National Chiao Tung University
Department of Electronics Engineering and Institute of Electronics
關鍵字: follicle-stimulating hormone;chemosensor;field effect transistor;microfluidic chip;diagnosis
公開日期: 24-十月-2018
摘要: The follicle-stimulating hormone (FSH) is a hormone that belongs to a family of glycoprotein hormones. Determination of FSH can help in interpreting various factors that include physiology of the reproductive system, fertility maintenance, and identification or treatment of reproductive disorders. Sialic acids are derivatives of neuraminic acids with negative charges, present at the end of the sugar chains and further linked to the cell surfaces and glycoproteins. The direct measurement of FSH in a human body can be recorded by developing a sensor probe that responds particularly to sialic acids over the other hormones. However, existing diagnostic methods still suffer from many difficulties in terms of complicated handling techniques, expensive instrumentation, etc. Development of accurate, rapid, and low-cost FSH detection chemosensors is important to meet these demands. Herein, we utilized a novel sensing method for accurate and fast FSH detection using a metal-oxide semiconductor silicon nanowire field effect transistor (SiNW-FET) device. This is the first report to demonstrate the boronic acid-functionalized SiNW-FET device in FSH detection. FSH detection has been successfully determined using an assay buffer solution with 0.72 fM detection limit as well as using 20% serum with 1.1. fM detection limit. We also investigated the specificity with other gonadotropins/glycosylated serum proteins. The current measurements on FSH concentrations at different time intervals were also studied. The sensitive, cheap, and miniaturized SiNW-FET device can serve as an effective sensing approach for rapid screening of FSH and menopause diagnosis.
URI: http://dx.doi.org/10.1021/acsami.8b11882
http://hdl.handle.net/11536/148387
ISSN: 1944-8244
DOI: 10.1021/acsami.8b11882
期刊: ACS APPLIED MATERIALS & INTERFACES
Volume: 10
起始頁: 36120
結束頁: 36127
顯示於類別:期刊論文