標題: | Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars |
作者: | Lin, Yi-Ying Chien, Yueh Chuang, Jen-Hua Chang, Chia-Ching Yang, Yi-Ping Lai, Ying-Hsiu Lo, Wen-Liang Chien, Ke-Hung Huo, Teh-Ia Wang, Chien-Ying 交大名義發表 生物科技學系 National Chiao Tung University Department of Biological Science and Technology |
關鍵字: | polydimethylsiloxane;graphene oxide;micropillar;retinal pigment epithelial cells |
公開日期: | 1-九月-2018 |
摘要: | Several efforts have been made on the development of bioscaffolds including the polydimethylsiloxane (PDMS) elastomer for supporting cell growth into stable sheets. However, PDMS has several disadvantages, such as intrinsic surface hydrophobicity and mechanical strength. Herein, we generated a novel PDMS-based biomimetic membrane by sequential modifications of the PMDS elastomer with graphene oxide (GO) and addition of a hexagonal micropillar structure at the bottom of the biomembrane. GO was initially homogenously mixed with pure PDMS and then was further coated onto the upper surface of the resultant PDMS. The elastic modulus and hydrophilicity were significantly improved by such modifications. In addition, the development of hexagonal micropillars with smaller diameters largely improved the ion permeability and increased the motion resistance. We further cultured retinal pigment epithelial (RPE) cells on the surface of this modified PDMS biomembrane and assayed its biocompatibility. Remarkably, the GO incorporation and coating exhibited beneficial effect on the cell growth and the new formation of tight junctions in RPE cells. Taken together, this GO-modified PDMS scaffold with polyhexagonal micropillars may be utilized as an ideal cell sheet and adaptor for cell cultivation and can be used in vivo for the transplantation of cells such as RPE cells. |
URI: | http://dx.doi.org/10.3390/ijms19092517 http://hdl.handle.net/11536/148454 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms19092517 |
期刊: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
Volume: | 19 |
顯示於類別: | 期刊論文 |