標題: Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars
作者: Lin, Yi-Ying
Chien, Yueh
Chuang, Jen-Hua
Chang, Chia-Ching
Yang, Yi-Ping
Lai, Ying-Hsiu
Lo, Wen-Liang
Chien, Ke-Hung
Huo, Teh-Ia
Wang, Chien-Ying
交大名義發表
生物科技學系
National Chiao Tung University
Department of Biological Science and Technology
關鍵字: polydimethylsiloxane;graphene oxide;micropillar;retinal pigment epithelial cells
公開日期: 1-九月-2018
摘要: Several efforts have been made on the development of bioscaffolds including the polydimethylsiloxane (PDMS) elastomer for supporting cell growth into stable sheets. However, PDMS has several disadvantages, such as intrinsic surface hydrophobicity and mechanical strength. Herein, we generated a novel PDMS-based biomimetic membrane by sequential modifications of the PMDS elastomer with graphene oxide (GO) and addition of a hexagonal micropillar structure at the bottom of the biomembrane. GO was initially homogenously mixed with pure PDMS and then was further coated onto the upper surface of the resultant PDMS. The elastic modulus and hydrophilicity were significantly improved by such modifications. In addition, the development of hexagonal micropillars with smaller diameters largely improved the ion permeability and increased the motion resistance. We further cultured retinal pigment epithelial (RPE) cells on the surface of this modified PDMS biomembrane and assayed its biocompatibility. Remarkably, the GO incorporation and coating exhibited beneficial effect on the cell growth and the new formation of tight junctions in RPE cells. Taken together, this GO-modified PDMS scaffold with polyhexagonal micropillars may be utilized as an ideal cell sheet and adaptor for cell cultivation and can be used in vivo for the transplantation of cells such as RPE cells.
URI: http://dx.doi.org/10.3390/ijms19092517
http://hdl.handle.net/11536/148454
ISSN: 1422-0067
DOI: 10.3390/ijms19092517
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 19
顯示於類別:期刊論文