完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Sie, Yong Da | en_US |
dc.contributor.author | Chang, Chia-Yuan | en_US |
dc.contributor.author | Lin, Chun-Yu | en_US |
dc.contributor.author | Chang, Nan-Shan | en_US |
dc.contributor.author | Campagnola, Paul J. | en_US |
dc.contributor.author | Chen, Shean-Jen | en_US |
dc.date.accessioned | 2019-04-02T05:58:08Z | - |
dc.date.available | 2019-04-02T05:58:08Z | - |
dc.date.issued | 2018-11-01 | en_US |
dc.identifier.issn | 1083-3668 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1117/1.JBO.23.11.116502 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/148570 | - |
dc.description.abstract | Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer wide-field optical sectioning with an axial excitation confinement of a few microns. To improve the axial confinement of TFMPEM, a binary computer-generated Fourier hologram (CGFH) via a digital-micromirror-device (DMD) was implemented to intrinsically improve the axial confinement by filling the back-focal aperture of the objective lens. Experimental results show that the excitation focal volume can be condensed and the axial confinement improved about 24% according to the DMD holography. In addition, pseudouniform MPE can be achieved using two complementary CGFHs with rapid pulse-width modulation switching via the DMD. Furthermore, bioimaging of CV-1 in origin with SV40 genes-7 cells demonstrates that the TFMPEM with binary DMD holography can improve image quality by enhancing axial excitation confinement and rejecting out-of-focus excitation. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE) | en_US |
dc.language.iso | en_US | en_US |
dc.subject | ultrafast lasers | en_US |
dc.subject | fluorescence microscopy | en_US |
dc.subject | medical and biological imaging | en_US |
dc.subject | nonlinear microscopy | en_US |
dc.title | Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1117/1.JBO.23.11.116502 | en_US |
dc.identifier.journal | JOURNAL OF BIOMEDICAL OPTICS | en_US |
dc.citation.volume | 23 | en_US |
dc.contributor.department | 光電學院 | zh_TW |
dc.contributor.department | College of Photonics | en_US |
dc.identifier.wosnumber | WOS:000452357900011 | en_US |
dc.citation.woscount | 0 | en_US |
顯示於類別: | 期刊論文 |