標題: Photoautotrophic synthesis of butyrate by metabolically engineered cyanobacteria
作者: Lai, Martin J.
Lan, Ethan I.
生物科技學系
Department of Biological Science and Technology
關鍵字: butyrate kinase;butyric acid;cyanobacteria;metabolic engineering;phosphotransbutyrylase;thioesterase
公開日期: 1-Apr-2019
摘要: Direct conversion of carbon dioxide into chemicals using engineered autotrophic microorganisms offers a potential solution for both sustainability and carbon mitigation. Butyrate is an important chemical used in various industries, including fragrance, food, and plastics. A model cyanobacterium Synechococcus elongatus PCC 7942 was engineered for the direct photosynthetic conversion of CO2 to butyrate. An engineered Clostridium Coenzyme A (CoA)-dependent pathway leading to the synthesis of butyryl-CoA, the precursor to butyrate, was introduced into S. elongatus PCC 7942. Two CoA removal strategies were then individually coupled to the modified CoA-dependent pathway to yield butyrate production. Similar results were observed between the two CoA removal strategies. The best butyrate producing strain of S. elongatus resulted in an observed butyrate titer of 750 mg/L and a cumulative titer of 1.1 g/L. These results demonstrated the feasibility of photosynthetic butyrate production and expanded the chemical repertoire accessible for production by photoautotrophs.
URI: http://dx.doi.org/10.1002/bit.26903
http://hdl.handle.net/11536/148951
ISSN: 0006-3592
DOI: 10.1002/bit.26903
期刊: BIOTECHNOLOGY AND BIOENGINEERING
Volume: 116
起始頁: 893
結束頁: 903
Appears in Collections:Articles