標題: Affinity capture of uropathogenic Escherichia coli using pigeon ovalbumin-bound Fe3O4@Al2O3 magnetic nanoparticles
作者: Liu, Jr-Chi
Tsai, Pei-Jane
Lee, Yuan C.
Chen, Yu-Chie
應用化學系
Department of Applied Chemistry
公開日期: 15-Jul-2008
摘要: Escherichia coli and Staphylococcus saprophyticus are the most common causes of urinary tract infections, with 80% of these infections caused by uropathogenic E. coli. Because the P fimbriae of E. coli have specificity toward Gal(alpha 1-4)Gal beta units, pigeon ovalbumin (POA), whose structure contains terminal Gal(alpha 1-4)Gal beta moieties, was used as a probe for interaction with P fimbriated E. coli. The functional affinity probes for these bacteria by immobilizing POA-a phosphoprotein-onto the surface of magnetic iron oxide nanoparticles (NPs) coated with alumina (Fe3O4@Al2O3), using the phosphate units of POA as linking groups for the formation of phosphate-alumina complexes. The immobilization process occurred within 30 s when performing the reaction under microwave heating. The magnetic POA-Fe3O4@Al2O3 NPs generated using this facile approach exhibited specificity toward P fimbriated E. coli. The bacteria targeted by the affinity probes were characterized by matrix-assisted laser desorption/ionization mass spectrometry. The detection limit toward uropathogenic bacteria when using this approach was similar to 9.60 x 10(4) cfu/mL (0.5 mL).
URI: http://dx.doi.org/10.1021/ac800487v
http://hdl.handle.net/11536/149468
ISSN: 0003-2700
DOI: 10.1021/ac800487v
期刊: ANALYTICAL CHEMISTRY
Volume: 80
起始頁: 5425
結束頁: 5432
Appears in Collections:Articles