標題: MAL-PDT inhibits oral precancerous cells and lesions via autophagic cell death
作者: Wang, Yen-Yun
Chen, Yuk-Kwan
Hu, Chu-Sung
Xiao, Ling-Yi
Huang, Wan-Ling
Chi, Tsung-Chen
Cheng, Kuang-Hung
Wang, Yun-Ming
Yuan, Shyng-Shiou F.
生物科技學系
Department of Biological Science and Technology
關鍵字: autophagy;methyl aminolevulinate;oral cancer;oral precancerous lesion;photodynamic therapy
公開日期: 1-Apr-2019
摘要: Background Oral cancer is a common cancer with a high mortality rate. While surgery is the most effective treatment for oral cancer, it frequently causes deformity and dysfunction in the orofacial region. In this study, methyl aminolevulinate photodynamic therapy (MAL-PDT) as a prevention tool against progression of precancerous lesion to oral cancer was explored. Methods For in vitro studies, we evaluated the effects of MAL-PDT on viability of DOK oral precancerous cells by XTT, cell morphology by TEM, and intracellular signaling pathways by flow cytometry, Western blotting, and immunofluorescence. For in vivo study, DMBA was used to induce oral precancerous lesions in hamsters followed by MAL-PDT treatment. We measured tumor size and body weight weekly. After sacrifice, buccal pouch lesions were processed for H&E stain and immunohistochemistry analysis. Results MAL-PDT induced autophagic cell death in DOK oral precancerous cells. The autophagy-related markers LC3II and p62/SQSTM1 and autophagosome formation in DOK cells were increased after MAL-PDT treatment. In vivo, MetvixA (R)-PDT treatment decreased tumor growth and enhanced LC3II expression in hamster buccal pouch tumors induced by DMBA. Conclusions Our in vitro and in vivo results suggest that MAL-PDT may provide an effective therapy for oral precancerous lesions through induction of autophagic cell death.
URI: http://dx.doi.org/10.1111/odi.13036
http://hdl.handle.net/11536/151611
ISSN: 1354-523X
DOI: 10.1111/odi.13036
期刊: ORAL DISEASES
Volume: 25
Issue: 3
起始頁: 758
結束頁: 771
Appears in Collections:Articles