標題: 3D pH MEASUREMENT FOR CELL CULTURE USING CT SCAN
作者: Higashino, Nobuya
Takayama, Toshio
Ito, Hiroaki
Horade, Mitsuhiro
Tsai, Chia-Hung Dylan
Kaneko, Makoto
機械工程學系
Department of Mechanical Engineering
公開日期: 1-Jan-2017
摘要: In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.
URI: http://hdl.handle.net/11536/151742
ISBN: 978-1-5386-3315-1
ISSN: 2474-378X
期刊: 2017 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)
起始頁: 0
結束頁: 0
Appears in Collections:Conferences Paper