標題: | 3D pH MEASUREMENT FOR CELL CULTURE USING CT SCAN |
作者: | Higashino, Nobuya Takayama, Toshio Ito, Hiroaki Horade, Mitsuhiro Tsai, Chia-Hung Dylan Kaneko, Makoto 機械工程學系 Department of Mechanical Engineering |
公開日期: | 1-Jan-2017 |
摘要: | In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance. |
URI: | http://hdl.handle.net/11536/151742 |
ISBN: | 978-1-5386-3315-1 |
ISSN: | 2474-378X |
期刊: | 2017 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS) |
起始頁: | 0 |
結束頁: | 0 |
Appears in Collections: | Conferences Paper |