標題: Essential Role of Visfatin in Lipopolysaccharide and Colon Ascendens Stent Peritonitis-Induced Acute Lung Injury
作者: Lee, Yi-Chen
Lin, Chun-Yu
Chen, Yen-Hsu
Chiu, Wen-Chin
Wang, Yen-Yun
Hsu, Chin
Hu, Stephen Chu-Sung
Su, Yu-Han
Yuan, Shyng-Shiou F.
交大名義發表
生醫工程研究所
National Chiao Tung University
Institute of Biomedical Engineering
關鍵字: acute lung injury;visfatin;FK866
公開日期: 4-Apr-2019
摘要: Acute lung injury (ALI) is a life-threatening syndrome characterized by acute and severe hypoxemic respiratory failure. Visfatin, which is known as an obesity-related cytokine with pro-inflammatory activities, plays a role in regulation of inflammatory cytokines. The mechanisms of ALI remain unclear in critically ill patients. Survival in ALI patients appear to be influenced by the stress generated by mechanical ventilation and by ALI-associated factors that initiate the inflammatory response. The objective for this study was to understand the mechanisms of how visfatin regulates inflammatory cytokines and promotes ALI. The expression of visfatin was evaluated in ALI patients and mouse sepsis models. Moreover, the underlying mechanisms were investigated using human bronchial epithelial cell lines, BEAS-2B and NL-20. An increase of serum visfatin was discovered in ALI patients compared to normal controls. Results from hematoxylin and eosin (H&E) and immunohistochemistry staining also showed that visfatin protein was upregulated in mouse sepsis models. Moreover, lipopolysaccharide (LPS) induced visfatin expression, activated the STAT3/NF kappa B pathway, and increased the expression of pro-inflammatory cytokines, including IL1-beta, IL-6, and TNF-alpha in human bronchial epithelial cell lines NL-20 and BEAS-2B. Co-treatment of visfatin inhibitor FK866 reversed the activation of the STAT3/NF kappa B pathway and the increase of pro-inflammatory cytokines induced by LPS. Our study provides new evidence for the involvement of visfatin and down-stream events in acute lung injury. Further studies are required to confirm whether the anti-visfatin approaches can improve ALI patient survival by alleviating the pro-inflammatory process.
URI: http://dx.doi.org/10.3390/ijms20071678
http://hdl.handle.net/11536/151968
ISSN: 1422-0067
DOI: 10.3390/ijms20071678
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 20
Issue: 7
起始頁: 0
結束頁: 0
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