標題: Enterotype-based Analysis of Gut Microbiota along the Conventional Adenoma-Carcinoma Colorectal Cancer Pathway
作者: Yang, Tzu-Wei
Lee, Wei-Hsiang
Tu, Siang-Jyun
Huang, Wei-Chih
Chen, Hui-Mei
Sun, Ting-Hsuan
Tsai, Ming-Chang
Wang, Chi-Chih
Chen, Hsuan-Yi
Huang, Chi-Chou
Shiu, Bei-Hao
Yang, Tzu-Ling
Huang, Hsin-Tzu
Chou, Yu-Pao
Chou, Chih-Hung
Huang, Ya-Rong
Sun, Yi-Run
Liang, Chao
Lin, Feng-Mao
Ho, Shinn-Ying
Chen, Wen-Liang
Yang, Shun-Fa
Ueng, Kwo-Chang
Huang, Hsien-Da
Huang, Chien-Ning
Jong, Yuh-Jyh
Lin, Chun-Che
交大名義發表
生物科技學院
National Chiao Tung University
College of Biological Science and Technology
公開日期: 29-Jul-2019
摘要: The dysbiosis of human gut microbiota is strongly associated with the development of colorectal cancer (CRC). The dysbiotic features of the transition from advanced polyp to early-stage CRC are largely unknown. We performed a 16S rRNA gene sequencing and enterotype-based gut microbiota analysis study. In addition to Bacteroides-and Prevotella-dominated enterotypes, we identified an Escherichia-dominated enterotype. We found that the dysbiotic features of CRC were dissimilar in overall samples and especially Escherichia-dominated enterotype. Besides a higher abundance of Fusobacterium, Enterococcus, and Aeromonas in all CRC faecal microbiota, we found that the most notable characteristic of CRC faecal microbiota was a decreased abundance of potential beneficial butyrate-producing bacteria. Notably, Oscillospira was depleted in the transition from advanced adenoma to stage 0 CRC, whereas Haemophilus was depleted in the transition from stage 0 to early-stage CRC. We further identified 7 different CAGs by analysing bacterial clusters. The abundance of microbiota in cluster 3 significantly increased in the CRC group, whereas that of cluster 5 decreased. The abundance of both cluster 5 and cluster 7 decreased in the Escherichia-dominated enterotype of the CRC group. We present the first enterotype-based faecal microbiota analysis. The gut microbiota of colorectal neoplasms can be influenced by its enterotype.
URI: http://dx.doi.org/10.1038/s41598-019-45588-z
http://hdl.handle.net/11536/152678
ISSN: 2045-2322
DOI: 10.1038/s41598-019-45588-z
期刊: SCIENTIFIC REPORTS
Volume: 9
起始頁: 0
結束頁: 0
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