標題: Intelligent whole-blood imaging flow cytometry for simple, rapid, and cost-effective drug-susceptibility testing of leukemia
作者: Kobayashi, Hirofumi
Lei, Cheng
Wu, Yi
Huang, Chun-Jung
Yasumoto, Atsushi
Jona, Masahiro
Li, Wenxuan
Wu, Yunzhao
Yalikun, Yaxiaer
Jiang, Yiyue
Guo, Baoshan
Sun, Chia-Wei
Tanaka, Yo
Yamada, Makoto
Yatomi, Yutaka
Goda, Keisuke
光電工程學系
Department of Photonics
公開日期: 21-Aug-2019
摘要: Drug susceptibility (also called chemosensitivity) is an important criterion for developing a therapeutic strategy for various cancer types such as breast cancer and leukemia. Recently, functional assays such as high-content screening together with genomic analysis have been shown to be effective for predicting drug susceptibility, but their clinical applicability is poor since they are time-consuming (several days long), labor-intensive, and costly. Here we present a highly simple, rapid, and cost-effective liquid biopsy for ex vivo drug-susceptibility testing of leukemia. The method is based on an extreme-throughput (>1 million cells per second), label-free, whole-blood imaging flow cytometer with a deep convolutional autoencoder, enabling image-based identification of the drug susceptibility of every single white blood cell in whole blood within 24 hours by simply flowing a drug-treated whole blood sample as little as 500 mu L into the imaging flow cytometer without labeling. Our results show that the method accurately evaluates the drug susceptibility of white blood cells from untreated patients with acute lymphoblastic leukemia. Our method holds promise for affordable precision medicine.
URI: http://dx.doi.org/10.1039/c8lc01370e
http://hdl.handle.net/11536/152731
ISSN: 1473-0197
DOI: 10.1039/c8lc01370e
期刊: LAB ON A CHIP
Volume: 19
Issue: 16
起始頁: 2688
結束頁: 2698
Appears in Collections:Articles