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dc.contributor.authorLee, Meng-Shiueen_US
dc.contributor.authorHsu, Wensyangen_US
dc.contributor.authorHuang, Hong-Yuanen_US
dc.contributor.authorTseng, Hsueh-Yangen_US
dc.contributor.authorLee, Chia-Tungen_US
dc.contributor.authorHsu, Chung-Yuen_US
dc.contributor.authorShieh, Yi-Chuehen_US
dc.contributor.authorWang, Shih-Hungen_US
dc.contributor.authorYao, Da-Jengen_US
dc.contributor.authorLiu, Cheng-Hsienen_US
dc.date.accessioned2020-03-02T03:23:30Z-
dc.date.available2020-03-02T03:23:30Z-
dc.date.issued2020-02-15en_US
dc.identifier.issn0956-5663en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2019.111851en_US
dc.identifier.urihttp://hdl.handle.net/11536/153772-
dc.description.abstractThe measurement of growth factors released in a culture medium is considered to be an attractive non-invasive approach, apart from the embryo morphology, to identify the condition of an embryo development after fertilization in vitro (IVF), but the available embryo culture medium in the current method is only a few microlitres. This small sample volume, also of small concentration, makes difficult the application of a conventional detection method, such as an enzyme-linked immunosorbent assay (ELISA). A reliable detection of the growth factor from each embryo culture medium of such a small concentration hence remains a challenge. Here for the first time we report the results of measurement of not just one, but two, growth factors, human IL-1 beta and human TNF-alpha, from an individual droplet of embryo culture medium with a bead-based digital microfluidic chip. The required sample volume for a single measurement is only 520 nL; the total duration of the on-chip process is less than 40 min. Using the culture media of human embryos with normal morphologic features, we found that the concentrations of TNF-alpha change little from day 3 to day 5-6, but the concentrations of IL-1 beta for some embryos might double from day 3 to day 5-6. For other embryos even with similar normal morphologic features, some growth factors, such as IL-1 beta, might exhibit different expressions during the culture period. Those growth factors could serve to distinguish the development conditions of each embryo, not merely from an observation of embryo morphology.en_US
dc.language.isoen_USen_US
dc.subjectIn vitro fertilizationen_US
dc.subjectIVFen_US
dc.subjectCulture mediumen_US
dc.subjectGrowth factoren_US
dc.subjectDigital microfluidicen_US
dc.subjectImmunoassayen_US
dc.titleSimultaneous detection of two growth factors from human single-embryo culture medium by a bead-based digital microfluidic chipen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bios.2019.111851en_US
dc.identifier.journalBIOSENSORS & BIOELECTRONICSen_US
dc.citation.volume150en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department機械工程學系zh_TW
dc.contributor.departmentDepartment of Mechanical Engineeringen_US
dc.identifier.wosnumberWOS:000509635500033en_US
dc.citation.woscount0en_US
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