標題: Exposure to Mono-n-Butyl Phthalate in Women with Endometriosis and Its Association with the Biological Effects on Human Granulosa Cells
作者: Chou, Ya-Ching
Chen, Yu-Chun
Chen, Ming-Jer
Chang, Ching-Wen
Lai, Guan-Lin
Tzeng, Chii-Ruey
交大名義發表
生物科技學系
National Chiao Tung University
Department of Biological Science and Technology
關鍵字: phthalate;mono-n-butyl phthalate (MnBP);endometriosis;granulosa cells;mitochondrial membrane potential;anti-Mullerian hormone
公開日期: 1-Mar-2020
摘要: To study the association between urinary phthalate metabolite levels, endometriosis, and their effects on human granulosa cells, we recruited patients who underwent laparoscopy to confirm endometriosis (n = 123) and control patients (n = 78). Liquid chromatography-tandem mass spectrometry was used to measure the following five urinary phthalate metabolites: mono-n-butyl phthalate (MnBP), mono(2-ethylhexyl) phthalate, monobenzyl phthalate, mono(2-ethyl-5-oxo-hexyl) phthalate, and mono(2-ethyl-5-hydroxyhexyl) phthalate. Urinary MnBP levels were higher in patients with endometriosis than in controls after multivariable logistic regression including the number of deliveries, body mass index, and use of medicine as covariables. MnBP correlates with other phthalate metabolites. Previous studies found that endometriosis was a detrimental condition for granulosa cells. In our study, we observed whether MnBP affected granulosa cells. MnBP treatment altered the gene expression of BIRC5, BUB1B, CDC20, cyclin B1, IL-1 beta, TNF-alpha, inhibin-B, StAR, and P450ssc and attenuated the ratio of the mitochondrial membrane potential in human granulosa cells. Moreover, MnBP decreased the expression of the anti-Mullerian hormone. These findings suggest that MnBP concentration is associated with endometriosis and may affect the health and steroidogenesis of human granulosa cells.
URI: http://dx.doi.org/10.3390/ijms21051794
http://hdl.handle.net/11536/154103
DOI: 10.3390/ijms21051794
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 21
Issue: 5
起始頁: 0
結束頁: 0
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