標題: | Epigenetic Regulation of WNT3A Enhancer during Regeneration of Injured Cortical Neurons |
作者: | Chang, Chu-Yuan Hung, Jui-Hung Huang, Liang-Wei Li, Joye Fung, Ka Shing Kao, Cheng-Fu Chen, Linyi 資訊工程學系 Department of Computer Science |
關鍵字: | enhancer regulation;histone modification;neuronal regeneration;epigenome;transcriptional regulation;WNT3A |
公開日期: | 1-三月-2020 |
摘要: | Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration. |
URI: | http://dx.doi.org/10.3390/ijms21051891 http://hdl.handle.net/11536/154104 |
DOI: | 10.3390/ijms21051891 |
期刊: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
Volume: | 21 |
Issue: | 5 |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 期刊論文 |