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dc.contributor.authorChang, Chia-Yuanen_US
dc.contributor.authorLin, Chun-Yuen_US
dc.contributor.authorHovhannisyan, Vladimir A.en_US
dc.contributor.authorChen, Shean-Jenen_US
dc.date.accessioned2020-07-01T05:21:47Z-
dc.date.available2020-07-01T05:21:47Z-
dc.date.issued2019-01-01en_US
dc.identifier.isbn978-1-5106-2846-5en_US
dc.identifier.issn0277-786Xen_US
dc.identifier.urihttp://dx.doi.org/10.1117/12.2526908en_US
dc.identifier.urihttp://hdl.handle.net/11536/154470-
dc.description.abstractA developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 mu m to 1.5 mu m for a 50% improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanning-based TFMPM to 1.5 mu m from optimal 3.0 mu m of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.en_US
dc.language.isoen_USen_US
dc.subjectMedical and biological imagingen_US
dc.subjectmultiphoton processesen_US
dc.subjectnonlinear microscopyen_US
dc.titleImproved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illuminationen_US
dc.typeProceedings Paperen_US
dc.identifier.doi10.1117/12.2526908en_US
dc.identifier.journalADVANCES IN MICROSCOPIC IMAGING IIen_US
dc.citation.volume11076en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department光電學院zh_TW
dc.contributor.departmentCollege of Photonicsen_US
dc.identifier.wosnumberWOS:000535353000013en_US
dc.citation.woscount0en_US
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