標題: Enhancing induced pluripotent stem cell toward differentiation into functional cardiomyocytes
作者: Chien, Chian-Shiu
Wang, Chien-Ying
Leu, Hsin-Bang
Chien, Yueh
Yang, Yi-Ping
Wang, Chia-Lin
Tai, Hsiao-Yun
Ko, Yu-Ling
Tsai, Fu-Ting
Chou, Shih-Jie
Yu, Wen-Chung
Yang, Meng-Yin
交大名義發表
National Chiao Tung University
關鍵字: Cardiomyocyte;Induced pluripotent stem cell (iPSC);Myocardial differentiation
公開日期: 1-七月-2020
摘要: Background: Heart diseases, especially myocardial ischemia, remain one of the leading causes of mortality worldwide and usually result in irreparable cardiomyocyte damage and severe heart failure. Recent advances in induced pluripotent stem cell (iPSC) technologies for applied regenerative medicine and stem cell research, especially for iPSC-derived cardiomyocytes have increased the hope for heart repair. However, the driver molecules of myocardial differentiation and the functional reconstruction capacity of iPSC-derived cardiomyocytes are still questionable. Methods: Herein, we established a rapid differentiated platform that is involved in cardiomyogenic differentiation and maturation from iPSCs in vitro. Functional analysis is performed in miR-181a-transfected iPSC-derived cardiomyocyte (iPSC-cardio/miR-181a) under a time-lapse microscope. In addition, we calculated the beating area and frequency of iPSC-cardio/miR-181a cells in the presence of HCN4 shRNA or miR-181a SPONGE. Results: miR-181a enhanced the beating area and maintained the beating frequency of iPSC-derived cardiomyocytes by enhancing HCN4 expression. Conclusion: miR-181a would play a key role on maintaining proper beating function in iPSC-derived cardiomyocytes.
URI: http://dx.doi.org/10.1097/JCMA.0000000000000301
http://hdl.handle.net/11536/155183
ISSN: 1726-4901
DOI: 10.1097/JCMA.0000000000000301
期刊: JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
Volume: 83
Issue: 7
起始頁: 657
結束頁: 660
顯示於類別:期刊論文