標題: | Enhancing induced pluripotent stem cell toward differentiation into functional cardiomyocytes |
作者: | Chien, Chian-Shiu Wang, Chien-Ying Leu, Hsin-Bang Chien, Yueh Yang, Yi-Ping Wang, Chia-Lin Tai, Hsiao-Yun Ko, Yu-Ling Tsai, Fu-Ting Chou, Shih-Jie Yu, Wen-Chung Yang, Meng-Yin 交大名義發表 National Chiao Tung University |
關鍵字: | Cardiomyocyte;Induced pluripotent stem cell (iPSC);Myocardial differentiation |
公開日期: | 1-七月-2020 |
摘要: | Background: Heart diseases, especially myocardial ischemia, remain one of the leading causes of mortality worldwide and usually result in irreparable cardiomyocyte damage and severe heart failure. Recent advances in induced pluripotent stem cell (iPSC) technologies for applied regenerative medicine and stem cell research, especially for iPSC-derived cardiomyocytes have increased the hope for heart repair. However, the driver molecules of myocardial differentiation and the functional reconstruction capacity of iPSC-derived cardiomyocytes are still questionable. Methods: Herein, we established a rapid differentiated platform that is involved in cardiomyogenic differentiation and maturation from iPSCs in vitro. Functional analysis is performed in miR-181a-transfected iPSC-derived cardiomyocyte (iPSC-cardio/miR-181a) under a time-lapse microscope. In addition, we calculated the beating area and frequency of iPSC-cardio/miR-181a cells in the presence of HCN4 shRNA or miR-181a SPONGE. Results: miR-181a enhanced the beating area and maintained the beating frequency of iPSC-derived cardiomyocytes by enhancing HCN4 expression. Conclusion: miR-181a would play a key role on maintaining proper beating function in iPSC-derived cardiomyocytes. |
URI: | http://dx.doi.org/10.1097/JCMA.0000000000000301 http://hdl.handle.net/11536/155183 |
ISSN: | 1726-4901 |
DOI: | 10.1097/JCMA.0000000000000301 |
期刊: | JOURNAL OF THE CHINESE MEDICAL ASSOCIATION |
Volume: | 83 |
Issue: | 7 |
起始頁: | 657 |
結束頁: | 660 |
顯示於類別: | 期刊論文 |