標題: Humanized bispecific antibody (mPEG x HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer
作者: Cheng, Yi-An
Wu, Tung-Ho
Wang, Yun-Ming
Cheng, Tian-Lu
Chen, I-Ju
Lu, Yun-Chi
Chuang, Kuo-Hsiang
Wang, Chih-Kuang
Chen, Chiao-Yun
Lin, Rui-An
Chen, Huei-Jen
Liao, Tzu-Yi
Liu, En-Shuo
Chen, Fang-Ming
生物科技學系
Department of Biological Science and Technology
關鍵字: Bispecific antibody;PEGylated nanoparticle;Contrast agent;Multimodality image;Polyethylene glycol;Anti-PEG antibody;One-step formulation;Tumor specificity;Cancer image
公開日期: 27-八月-2020
摘要: Background: Developing a universal strategy to improve the specificity and sensitivity of PEGylated nanoaparticles (PEG-NPs) for assisting in the diagnosis of tumors is important in multimodality imaging. Here, we developed the anti-methoxypolyethylene glycol (mPEG) bispecific antibody (BsAb; mPEG x HER2), which has dual specificity for mPEG and human epidermal growth factor receptor 2 (HER2), with a diverse array of PEG-NPs to confer nanoparticles with HER2 specificity and stronger intensity. Result: We used a one-step formulation to rapidly modify the nanoprobes with mPEG x HER2 and optimized the modified ratio of BsAbs on several PEG-NPs (Lipo-DiR, SPIO, Qdot and AuNP). The alpha HER2/PEG-NPs could specifically target MCF7/HER2 cells (HER2(++)) but not MCF7/neo1 cells (HER2(+/-)). The alpha HER2/Lipo-DiR and alpha HER2/SPIO could enhance the sensitivity of untargeted PEG-NPs on MCF7/HER2 (HER2(++)). In in vivo imaging, alpha HER2/Lipo-DiR and alpha HER2/SPIO increased the specific targeting and enhanced PEG-NPs accumulation at 175% and 187% on 24 h, respectively, in HER2-overexpressing tumors. Conclusion: mPEG x HER2, therefore, provided a simple one-step formulation to confer HER2-specific targeting and enhanced sensitivity and contrast intensity on HER2 positive tumors for multimodality imaging.
URI: http://dx.doi.org/10.1186/s12951-020-00680-9
http://hdl.handle.net/11536/155354
DOI: 10.1186/s12951-020-00680-9
期刊: JOURNAL OF NANOBIOTECHNOLOGY
Volume: 18
Issue: 1
起始頁: 0
結束頁: 0
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