标题: | R432 is a key residue for the multiple functions of Ndt80p in Candida albicans |
作者: | Yang, Yun Liang Wang, Chih Wei Leaw, Shiang Ning Chang, Te Pin Wang, I. Chin Chen, Chia Geun Fan, Jen Chung Tseng, Kuo Yun Huang, Szu Hsuan Chen, Chih Yu Hsiao, Ting Yin Hsiung, Chao Agnes Chen, Chiung Tong Hsiao, Chwan Deng Lo, Hsiu Jung 生物科技学系 分子医学与生物工程研究所 Department of Biological Science and Technology Institute of Molecular Medicine and Bioengineering |
关键字: | Candida albicans;Ndt80;Protein-DNA interaction;MSE;Binding assay |
公开日期: | 1-三月-2012 |
摘要: | Ndt80p is an important transcription modulator to various stress-response genes in Candida albicans, the most common human fungal pathogen in systemic infections. We found that Ndt80p directly regulated its target genes, such as YHB1, via the mid-sporulation element (MSE). Furthermore, the ndt80 (R432A) allele, with a reduced capability to bind MSE, failed to complement the defects caused by null mutations of NDT80. Thus, the R432 residue in the Ndt80p DNA-binding domain is involved in all tested functions, including cell separation, drug resistance, nitric oxide inactivation, germ tube formation, hyphal growth, and virulence. Hence, the importance of the R432 residue suggests a novel approach for designing new antifungal drugs by blocking the interaction between Ndt80p and its targets. |
URI: | http://dx.doi.org/10.1007/s00018-011-0849-5 http://hdl.handle.net/11536/15603 |
ISSN: | 1420-682X |
DOI: | 10.1007/s00018-011-0849-5 |
期刊: | CELLULAR AND MOLECULAR LIFE SCIENCES |
Volume: | 69 |
Issue: | 6 |
起始页: | 1011 |
结束页: | 1023 |
显示于类别: | Articles |
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