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dc.contributor.authorChao, Cheng-Hanen_US
dc.contributor.authorLi, Kun-Linen_US
dc.contributor.authorWu, Chung-Shuen_US
dc.contributor.authorLee, Cheng-Cheen_US
dc.contributor.authorChiang, Han-Pingen_US
dc.contributor.authorYang, Yuh-Shyongen_US
dc.contributor.authorPan, Tung-Mingen_US
dc.contributor.authorKo, Fu-Hsiangen_US
dc.date.accessioned2014-12-08T15:23:38Z-
dc.date.available2014-12-08T15:23:38Z-
dc.date.issued2012-06-01en_US
dc.identifier.issn1452-3981en_US
dc.identifier.urihttp://hdl.handle.net/11536/16524-
dc.description.abstractThe fluorescent marker of rhodamine B amine is successfully used to evaluate the immobilization capability onto silicon-based patterns fabricated by semiconductor manufacturing. Only the silicon dioxide surface, by means of fluorescent observation, can immobilize the rhodamine molecule by the sequential linkage of (3-aminopropyl) triethoxysilane (APTES) and glutaraldehyde. The phenol sulfotransferase enzyme is also successfully immobilized onto the silicon dioxide surface by the linking molecules of APTES and sulfosuccinimidyl 4-(N-maleimidomethyl)-cyclohezane-1-carboxylate in the home-made apparatus. The enzyme activity of the sulfotransferase is determined from the absorbance of 4-nitrophenol at 400 nm wavelength. The surface immobilized enzyme remains its activity for catalytic reaction at least 120-min duration. The surface saturation effect on the activity of immobilized enzyme is explained and ascribed to the surface diffusion effect of electric double layers. We can success control the surface immobilized enzyme by electric potential stress. The activity of enzyme is reduced under negative potential, while is enhanced under positive potential. The electric potential can induce the enzyme structure variation and modulate the enzyme activity due to the electrostatic effect.en_US
dc.language.isoen_USen_US
dc.subjectrhodamine B amineen_US
dc.subjectsurface immobilizationen_US
dc.subjectsulfotransferaseen_US
dc.subjectsurface diffusion modelen_US
dc.subjectenzyme activity modulationen_US
dc.titleSurface Effect of Assembling Enzyme and Modulation of Surface Enzyme Activity with Electric Potential Stressen_US
dc.typeArticleen_US
dc.identifier.journalINTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCEen_US
dc.citation.volume7en_US
dc.citation.issue6en_US
dc.citation.epage5100en_US
dc.contributor.department材料科學與工程學系zh_TW
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Materials Science and Engineeringen_US
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000305267400022-
dc.citation.woscount2-
Appears in Collections:Articles