Protein Engineering of Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase into Parkeol Synthase

doi:10.1021/ol302341h

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DC Field	Value	Language
dc.contributor.author	Liu, Yuan-Ting	en_US
dc.contributor.author	Hu, Tain-Chang	en_US
dc.contributor.author	Chang, Cheng-Hsiang	en_US
dc.contributor.author	Shie, Wen-Shiang	en_US
dc.contributor.author	Wu, Tung-Kung	en_US
dc.date.accessioned	2014-12-08T15:28:12Z	-
dc.date.available	2014-12-08T15:28:12Z	-
dc.date.issued	2012-10-19	en_US
dc.identifier.issn	1523-7060	en_US
dc.identifier.uri	http://dx.doi.org/10.1021/ol302341h	en_US
dc.identifier.uri	http://hdl.handle.net/11536/20423	-
dc.description.abstract	A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7(T384Y/Q450H/V4541), produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.	en_US
dc.language.iso	en_US	en_US
dc.title	Protein Engineering of Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase into Parkeol Synthase	en_US
dc.type	Article	en_US
dc.identifier.doi	10.1021/ol302341h	en_US
dc.identifier.journal	ORGANIC LETTERS	en_US
dc.citation.volume	14	en_US
dc.citation.issue	20	en_US
dc.citation.spage	5222	en_US
dc.citation.epage	5225	en_US
dc.contributor.department	生物科技學系	zh_TW
dc.contributor.department	Department of Biological Science and Technology	en_US
dc.identifier.wosnumber	WOS:000309951500015	-
dc.citation.woscount	1	-
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