完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Tseng, Te-Wei | en_US |
dc.contributor.author | Wu, June-Tai | en_US |
dc.contributor.author | Chen, Yu-Chie | en_US |
dc.contributor.author | Urban, Pawel L. | en_US |
dc.date.accessioned | 2014-12-08T15:28:54Z | - |
dc.date.available | 2014-12-08T15:28:54Z | - |
dc.date.issued | 2012-11-21 | en_US |
dc.identifier.issn | 1932-6203 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1371/journal.pone.0050258 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/20874 | - |
dc.description.abstract | In order to investigate the influence of light/dark cycle on the biosynthesis of metabolites during oogenesis, here we demonstrate a simple experimental protocol which combines in-vivo isotopic labeling of primary metabolites with mass spectrometric analysis of single eggs of fruit fly (Drosophila melanogaster). First, fruit flies were adapted to light/dark cycle using artificial white light. Second, female flies were incubated with an isotopically labeled sugar (C-13(6)-glucose) for 12 h - either during the circadian day or the circadian night, at light or at dark. Third, eggs were obtained from the incubated female flies, and analyzed individually by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS): this yielded information about the extent of labeling with carbon-13. Since the incorporation of carbon-13 to uridine diphosphate glucose (UDP-glucose) in fruit fly eggs is very fast, the labeling of this metabolite was used as an indicator of the biosynthesis of metabolites flies/eggs during 12-h periods, which correspond to circadian day or circadian night. The results reveal that once the flies adapted to the 12-h-light/12-h-dark cycle, the incorporation of carbon-13 to UDP-glucose present in fruit fly eggs was not markedly altered by an acute perturbation to this cycle. This effect may be due to a relationship between biosynthesis of primary metabolites in developing eggs and an alteration to the intake of the labeled substrate - possibly related to the change of the feeding habit. Overall, the study shows the possibility of using MALDI-MS in conjunction with isotopic labeling of small metazoans to unravel the influence of environmental cues on primary metabolism. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Isotope Label-Aided Mass Spectrometry Reveals the Influence of Environmental Factors on Metabolism in Single Eggs of Fruit Fly | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1371/journal.pone.0050258 | en_US |
dc.identifier.journal | PLOS ONE | en_US |
dc.citation.volume | 7 | en_US |
dc.citation.issue | 11 | en_US |
dc.citation.epage | en_US | |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | 應用化學系分子科學碩博班 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.contributor.department | Institute of Molecular science | en_US |
dc.identifier.wosnumber | WOS:000311821000207 | - |
dc.citation.woscount | 0 | - |
顯示於類別: | 期刊論文 |