完整後設資料紀錄
DC 欄位語言
dc.contributor.authorLu, Huai-Enen_US
dc.contributor.authorYang, Yao-Chenen_US
dc.contributor.authorChen, Sheng-Meien_US
dc.contributor.authorSu, Hong-Linen_US
dc.contributor.authorHuang, Pai-Chengen_US
dc.contributor.authorTsai, Ming-Songen_US
dc.contributor.authorWang, Tzu-Haoen_US
dc.contributor.authorTseng, Ching-Pingen_US
dc.contributor.authorHwang, Shiaw-Minen_US
dc.date.accessioned2014-12-08T15:29:30Z-
dc.date.available2014-12-08T15:29:30Z-
dc.date.issued2013-02-15en_US
dc.identifier.issn0014-4827en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.yexcr.2012.09.017en_US
dc.identifier.urihttp://hdl.handle.net/11536/21212-
dc.description.abstractDown syndrome (DS), or Trisomy 21 (121) syndrome, one of the most common chromosomal abnormalities, is caused by an extra duplication of chromosome 21. In studies of neuron development, experimental models based on human cells are considered to be the most desired and accurate for basic research. The generation of diseased induced pluripotetn stem (iPS) cell is a critical step in understanding the developmental stages of complex neuronal diseases. Here, we generated human DS iPS cell lines from second trimester amniotic fluid (AF) cells with 121 by co-expressing Yamanaka factors through lentiviral delivery and subsequently differentiated them into neuronal progenitor cells (NPCs) for further analyses. 121 AF-iPS cells were characterized for the expression of pluripotent markers and for their ability to differentiate into all three germ layers by forming embryoid bodies in vitro and teratomas in vivo. The 121 AF-iPS cells maintained their unique pattern of chromosomal karyotypes: three pairs of chromosome 21. The level of amyloid precursor protein was significantly increased in NPCs derived from 121 AF-iPS cells compared with NPCs from normal AF-iPS cells. The expression levels of miR-155 and miR-802 in 121 AF-iPS-NPCs were highly elevated in the presence of low expression of MeCP2. We observed that 121 iPS-NPCs generated fewer neurons compared with controls. 121 iPS-NPCs exhibit developmental defects during neurogenesis. Our findings suggest that 121 AF-iPS cells serve as a good source to further elucidate the impairment neurogenesis of DS and the onset of Alzheimer's disease. (C) 2012 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectDown syndromeen_US
dc.subjectTrisomy 21en_US
dc.subjectNeurogenesis impairmenten_US
dc.subjectInduced pluripotent stem cellsen_US
dc.subjectmiRNAen_US
dc.titleModeling neurogenesis impairment in down syndrome with induced pluripotent stem cells from Trisomy 21 amniotic fluid cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.yexcr.2012.09.017en_US
dc.identifier.journalEXPERIMENTAL CELL RESEARCHen_US
dc.citation.volume319en_US
dc.citation.issue4en_US
dc.citation.spage498en_US
dc.citation.epage505en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000315067800013-
dc.citation.woscount9-
顯示於類別:期刊論文


文件中的檔案:

  1. 000315067800013.pdf

若為 zip 檔案,請下載檔案解壓縮後,用瀏覽器開啟資料夾中的 index.html 瀏覽全文。