完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chien, C. C. | en_US |
dc.contributor.author | Tseng, P. Y. | en_US |
dc.contributor.author | Chen, H. H. | en_US |
dc.contributor.author | Hua, T. E. | en_US |
dc.contributor.author | Chen, S. T. | en_US |
dc.contributor.author | Chen, Y. Y. | en_US |
dc.contributor.author | Leng, W. H. | en_US |
dc.contributor.author | Wang, C. H. | en_US |
dc.contributor.author | Hwu, Y. | en_US |
dc.contributor.author | Yin, G. C. | en_US |
dc.contributor.author | Liang, K. S. | en_US |
dc.contributor.author | Chen, F. R. | en_US |
dc.contributor.author | Chu, Y. S. | en_US |
dc.contributor.author | Yeh, H. I. | en_US |
dc.contributor.author | Yang, Y. C. | en_US |
dc.contributor.author | Yang, C. S. | en_US |
dc.contributor.author | Zhang, G. L. | en_US |
dc.contributor.author | Je, J. H. | en_US |
dc.contributor.author | Margaritondo, G. | en_US |
dc.date.accessioned | 2014-12-08T15:30:02Z | - |
dc.date.available | 2014-12-08T15:30:02Z | - |
dc.date.issued | 2013-05-01 | en_US |
dc.identifier.issn | 0734-9750 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1016/j.biotechadv.2012.04.005 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/21505 | - |
dc.description.abstract | Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research. (C) 2012 Elsevier Inc. All rights reserved. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | X-ray microscopy | en_US |
dc.subject | Phase contrast radiology | en_US |
dc.subject | Fresnel phase zone plate | en_US |
dc.subject | Subcellular organelle | en_US |
dc.title | Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy | en_US |
dc.type | Article; Proceedings Paper | en_US |
dc.identifier.doi | 10.1016/j.biotechadv.2012.04.005 | en_US |
dc.identifier.journal | BIOTECHNOLOGY ADVANCES | en_US |
dc.citation.volume | 31 | en_US |
dc.citation.issue | 3 | en_US |
dc.citation.spage | 375 | en_US |
dc.citation.epage | 386 | en_US |
dc.contributor.department | 電子物理學系 | zh_TW |
dc.contributor.department | Department of Electrophysics | en_US |
dc.identifier.wosnumber | WOS:000317441900004 | - |
顯示於類別: | 會議論文 |