標題: AC electrokinetics assisted impedance biosensors for rapid bacteria detection
作者: Ho, Yi-Tse
Pan, Chun-Liang
Sheu, Jeng-Tzong
Wang, Yu-Kuo
Wu, Tung-Kung
材料科學與工程學系
Department of Materials Science and Engineering
公開日期: 2012
摘要: Early detection of living bacteria attracts a lot of attention in infection diseases applications, especially at low concentration. Nowadays, the detection of specific bacteria faces challenges including enhancement of trapping efficiency, and increase of sensor sensitivity and specificity. In this study, we improved the trapping efficiency and increased sensor sensitivity and specificity by using AC electrokinetics and selective modification of antibody on gold electrode in a new structure of gold electrode design. COMSOL simulations of AC electrokinetics include dielectrophoresis (DEP), and AC electroosmosis (ACEO) were performed on different electrode designs and bias configurations. The simulation results were consistent with the fluorescence observations showing that the bacteria were trapping selectively on gold electrodes. The impedance measurements for Vibrio parahaemolyticus (Vp) detection at 10(5) cfu/ml with AC elctrokinetics showed a nearly 90% change in normalized impedance change (NIC).
URI: http://hdl.handle.net/11536/21535
ISBN: 978-1-4673-2293-5
ISSN: 2163-4025
期刊: 2012 IEEE BIOMEDICAL CIRCUITS AND SYSTEMS CONFERENCE (BIOCAS): INTELLIGENT BIOMEDICAL ELECTRONICS AND SYSTEM FOR BETTER LIFE AND BETTER ENVIRONMENT
起始頁: 49
結束頁: 52
Appears in Collections:Conferences Paper