標題: | Resuscitation from experimental traumatic brain injury by magnolol therapy |
作者: | Wang, Che-Chuan Lin, Kao-Chang Lin, Bor-Shyh Chio, Chung-Ching Kuo, Jinn-Rung 影像與生醫光電研究所 Institute of Imaging and Biomedical Photonics |
關鍵字: | Traumatic brain injury;Magnolol;Free radical scavenger;Neuronal apoptosis;Transforming growth factor beta 1 |
公開日期: | 1-十月-2013 |
摘要: | Background: The purpose of the present study was to determine whether magnolol, a free radical scavenger, mitigates the deleterious effects of traumatic brain injury (TBI). Material and methods: Traumatic brain injuries were induced in anesthetized male Sprague-Dawley rats using fluid percussion, and the rats were divided into groups treated with magnolol (2 mg/kg, intravenously) or vehicle. A group of rats that did not undergo TBI induction was also studied as controls. Biomarkers of TBI, including glycerol and 2,3-dihydroxybenzoic acid, were evaluated by microdialysis. Infraction volume, extent of neuronal apoptosis, and antiapoptosis factor transforming growth factor beta 1 (TGF-beta 1) were also measured. Functional outcomes were assessed by motor assays. Results: Compared with the rats without TBI, the animals with TBI exhibited higher hippocampal glycerol and 2,3-dihydroxybenzoic acid. Relative to the vehicle-treated group, the magnolol-treated group showed decreased hippocampal levels of glycerol and hydroxyl radical levels. The magnolol-treated rats also exhibited decreased cerebral infarction volume and neuronal apoptosis and increased antiapoptosis-associated factor TGF-beta 1 expression. These effects were translated into improved motor function post TBI. Conclusions: Our results suggest that intravenous magnolol injection mitigates the deleterious effects of TBI in rats based on its potent free radical scavenging capability, and the mechanism of anti-neuronal apoptosis is partly due to an increase in TGF-beta 1 expression in the ischemic cortex. (C) 2013 Elsevier Inc. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.jss.2013.04.059 http://hdl.handle.net/11536/22738 |
ISSN: | 0022-4804 |
DOI: | 10.1016/j.jss.2013.04.059 |
期刊: | JOURNAL OF SURGICAL RESEARCH |
Volume: | 184 |
Issue: | 2 |
起始頁: | 1045 |
結束頁: | 1052 |
顯示於類別: | 期刊論文 |