標題: Submicron-grooved culture surface extends myotube length by forming parallel and elongated motif
作者: Yuan, Chen-Ching
Ma, Kuang-Jen
Li, Kuei-Chi
Chien, Hsi-Hsin
Lu, Huai-En
Tseng, Ching-Ping
Hwang, Shiaw-Min
生物科技學系
Department of Biological Science and Technology
關鍵字: biomechanics;biomedical materials;cellular transport;elongation;lithography;muscle;neurophysiology;polymers;tissue engineering;submicron-grooved culture surface;myotube length;elongated motif;parallel motif;cellular orientation;functional muscle fibres;submicron-imprint lithography;polystyrene plates;in-vitro striated myotubes;mouse muscle myoblast cells;parallel myotubes;co-culture submicron-groove-grown myotubes;neurotransmitter secreting cells;myotube formation;striated motifs;physiological functionality;muscle tissue engineering;skeletal muscle development;cell migration;cell maturation;cell growth
公開日期: 1-八月-2013
摘要: During skeletal muscle development, correct cellular orientation is vital to generate desired longitudinal contraction for functional muscle fibres. In this reported study, submicron-imprint lithography was used to generate submicron-grooved surfaces on polystyrene plates to induce striated myotubes in vitro. Mouse muscle myoblast cells cultured on a submicron-grooved surface migrated faster in a directionally uniform fashion; in comparison, cells cultured on a flat surface grew and migrated slower in indiscriminate directions. Subsequent maturation of the myoblast cells formed along the submicron-groove surface resulted in a tandem of parallel myotubes that were both longer and greater in circumference than in the case of the flat surface. In a functional test, the co-culture submicron-groove-grown myotubes with neurotransmitter secreting cells further demonstrated contraction abilities, suggesting submicron-groove-guided growth served to enhance myotube formation while retaining striated motifs and physiological functionality for muscle tissue engineering.
URI: http://dx.doi.org/10.1049/mnl.2013.0153
http://hdl.handle.net/11536/23045
ISSN: 1750-0443
DOI: 10.1049/mnl.2013.0153
期刊: MICRO & NANO LETTERS
Volume: 8
Issue: 8
起始頁: 440
結束頁: 444
顯示於類別:期刊論文


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