標題: | Submicron-grooved culture surface extends myotube length by forming parallel and elongated motif |
作者: | Yuan, Chen-Ching Ma, Kuang-Jen Li, Kuei-Chi Chien, Hsi-Hsin Lu, Huai-En Tseng, Ching-Ping Hwang, Shiaw-Min 生物科技學系 Department of Biological Science and Technology |
關鍵字: | biomechanics;biomedical materials;cellular transport;elongation;lithography;muscle;neurophysiology;polymers;tissue engineering;submicron-grooved culture surface;myotube length;elongated motif;parallel motif;cellular orientation;functional muscle fibres;submicron-imprint lithography;polystyrene plates;in-vitro striated myotubes;mouse muscle myoblast cells;parallel myotubes;co-culture submicron-groove-grown myotubes;neurotransmitter secreting cells;myotube formation;striated motifs;physiological functionality;muscle tissue engineering;skeletal muscle development;cell migration;cell maturation;cell growth |
公開日期: | 1-八月-2013 |
摘要: | During skeletal muscle development, correct cellular orientation is vital to generate desired longitudinal contraction for functional muscle fibres. In this reported study, submicron-imprint lithography was used to generate submicron-grooved surfaces on polystyrene plates to induce striated myotubes in vitro. Mouse muscle myoblast cells cultured on a submicron-grooved surface migrated faster in a directionally uniform fashion; in comparison, cells cultured on a flat surface grew and migrated slower in indiscriminate directions. Subsequent maturation of the myoblast cells formed along the submicron-groove surface resulted in a tandem of parallel myotubes that were both longer and greater in circumference than in the case of the flat surface. In a functional test, the co-culture submicron-groove-grown myotubes with neurotransmitter secreting cells further demonstrated contraction abilities, suggesting submicron-groove-guided growth served to enhance myotube formation while retaining striated motifs and physiological functionality for muscle tissue engineering. |
URI: | http://dx.doi.org/10.1049/mnl.2013.0153 http://hdl.handle.net/11536/23045 |
ISSN: | 1750-0443 |
DOI: | 10.1049/mnl.2013.0153 |
期刊: | MICRO & NANO LETTERS |
Volume: | 8 |
Issue: | 8 |
起始頁: | 440 |
結束頁: | 444 |
顯示於類別: | 期刊論文 |