完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yuan, Chen-Ching | en_US |
dc.contributor.author | Ma, Kuang-Jen | en_US |
dc.contributor.author | Li, Kuei-Chi | en_US |
dc.contributor.author | Chien, Hsi-Hsin | en_US |
dc.contributor.author | Lu, Huai-En | en_US |
dc.contributor.author | Tseng, Ching-Ping | en_US |
dc.contributor.author | Hwang, Shiaw-Min | en_US |
dc.date.accessioned | 2014-12-08T15:33:06Z | - |
dc.date.available | 2014-12-08T15:33:06Z | - |
dc.date.issued | 2013-08-01 | en_US |
dc.identifier.issn | 1750-0443 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1049/mnl.2013.0153 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/23045 | - |
dc.description.abstract | During skeletal muscle development, correct cellular orientation is vital to generate desired longitudinal contraction for functional muscle fibres. In this reported study, submicron-imprint lithography was used to generate submicron-grooved surfaces on polystyrene plates to induce striated myotubes in vitro. Mouse muscle myoblast cells cultured on a submicron-grooved surface migrated faster in a directionally uniform fashion; in comparison, cells cultured on a flat surface grew and migrated slower in indiscriminate directions. Subsequent maturation of the myoblast cells formed along the submicron-groove surface resulted in a tandem of parallel myotubes that were both longer and greater in circumference than in the case of the flat surface. In a functional test, the co-culture submicron-groove-grown myotubes with neurotransmitter secreting cells further demonstrated contraction abilities, suggesting submicron-groove-guided growth served to enhance myotube formation while retaining striated motifs and physiological functionality for muscle tissue engineering. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | biomechanics | en_US |
dc.subject | biomedical materials | en_US |
dc.subject | cellular transport | en_US |
dc.subject | elongation | en_US |
dc.subject | lithography | en_US |
dc.subject | muscle | en_US |
dc.subject | neurophysiology | en_US |
dc.subject | polymers | en_US |
dc.subject | tissue engineering | en_US |
dc.subject | submicron-grooved culture surface | en_US |
dc.subject | myotube length | en_US |
dc.subject | elongated motif | en_US |
dc.subject | parallel motif | en_US |
dc.subject | cellular orientation | en_US |
dc.subject | functional muscle fibres | en_US |
dc.subject | submicron-imprint lithography | en_US |
dc.subject | polystyrene plates | en_US |
dc.subject | in-vitro striated myotubes | en_US |
dc.subject | mouse muscle myoblast cells | en_US |
dc.subject | parallel myotubes | en_US |
dc.subject | co-culture submicron-groove-grown myotubes | en_US |
dc.subject | neurotransmitter secreting cells | en_US |
dc.subject | myotube formation | en_US |
dc.subject | striated motifs | en_US |
dc.subject | physiological functionality | en_US |
dc.subject | muscle tissue engineering | en_US |
dc.subject | skeletal muscle development | en_US |
dc.subject | cell migration | en_US |
dc.subject | cell maturation | en_US |
dc.subject | cell growth | en_US |
dc.title | Submicron-grooved culture surface extends myotube length by forming parallel and elongated motif | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1049/mnl.2013.0153 | en_US |
dc.identifier.journal | MICRO & NANO LETTERS | en_US |
dc.citation.volume | 8 | en_US |
dc.citation.issue | 8 | en_US |
dc.citation.spage | 440 | en_US |
dc.citation.epage | 444 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000326458700012 | - |
dc.citation.woscount | 0 | - |
顯示於類別: | 期刊論文 |