標題: ULTRA SENSITIVE DETECTION OF ENETEROVIRUS 71 BY MODIFIED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY
作者: Tseng, Shin-Hua
Tseng, Dion T.
Lee, Tzu-Cheng
Cheng, Tsai-Mu
Yang, Jyh-Yuan
Hsieh, Ruo-Yu
Tsai, Chuan-Mei
Chang, Chia-Ching
生醫與生物工程研究中心
Center for Biomedical and Biological Engineering
公開日期: 2010
摘要: The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (similar to sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.
URI: http://hdl.handle.net/11536/23333
ISBN: 978-0-7918-4392-5
期刊: NEMB2010: PROCEEDINGS OF THE ASME FIRST GLOBAL CONGRESS ON NANOENGINEERING FOR MEDICINE AND BIOLOGY - 2010
起始頁: 27
結束頁: 28
Appears in Collections:Conferences Paper