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dc.contributor.authorLin, Kuen-Youen_US
dc.contributor.authorChang, Wei-Tienen_US
dc.contributor.authorLai, Yu-Chengen_US
dc.contributor.authorLiau, Ianen_US
dc.date.accessioned2014-12-08T15:34:59Z-
dc.date.available2014-12-08T15:34:59Z-
dc.date.issued2014-02-18en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://dx.doi.org/10.1021/ac403877hen_US
dc.identifier.urihttp://hdl.handle.net/11536/23780-
dc.description.abstractGiven the high mortality in patients with cardiovascular diseases and the life-threatening consequences of drugs with unforeseen adverse effects on hearts, a critical evaluation of the pharmacological response of cardiovascular function on model animals is important especially in the early stages of drug development. We report a proof-of-principle study to demonstrate the utility of zebrafish as an analytical platform to predict the cardiac response of new drugs or chemicals on human beings. With pseudodynamic 3D imaging, we derive individual parameters that are central to the cardiac function of zebrafish, including the ventricular stroke volume, ejection fraction, cardiac output, heart rate, diastolic filling function, and ventricular mass. We evaluate both inotropic and chronotropic responses of the heart of zebrafish treated with drugs that are commonly prescribed and possess varied known cardiac activities. We reveal deranged cardiac function of a zebrafish model of cardiomyopathy induced with a cardiotoxic drug. The cardiac function of zebrafish exhibits a pharmacological response similar to that of human beings. We compare also cardiac parameters obtained in this work with those derived with conventional 2D approximation and show that the latter tends to overestimate the cardiac parameters and produces results of greater variation. In view of the growing interest of using zebrafish in both fundamental and translational biomedical research, we envisage that our approach should benefit not only contemporary pharmaceutical development but also exploratory research such as gene, stem cell, or regenerative therapies targeting congenital or acquired heart diseases.en_US
dc.language.isoen_USen_US
dc.titleToward Functional Screening of Cardioactive and Cardiotoxic Drugs with Zebrafish in Vivo Using Pseudodynamic Three-Dimensional Imagingen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac403877hen_US
dc.identifier.journalANALYTICAL CHEMISTRYen_US
dc.citation.volume86en_US
dc.citation.issue4en_US
dc.citation.spage2213en_US
dc.citation.epage2220en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000331775600039-
dc.citation.woscount1-
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