標題: | Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions |
作者: | Chien, Wei-Ting Liang, Chien-Fu Yu, Ching-Ching Lin, Chien-Hung Li, Si-Peng Primadona, Indah Chen, Yu-Ju Mong, Kwok Kong T. Lin, Chun-Cheng 應用化學系 Department of Applied Chemistry |
公開日期: | 2014 |
摘要: | A simple and efficient protocol for the preparative-scale synthesis of various lengths of oligo-N-acetyllactosamine (oligo-LacNAc) and its multi-sialylated extensions is described. The strategy utilizes one thermophilic bacterial thymidylyltransferase (RmlA) coupled with corresponding sugar-1-phosphate kinases to generate two uridine diphosphate sugars, UDP-galactose and UDP-N-acetylglucosamine. By incorporating glycosyltransferases, oligo-LacNAcs and their sialylated analogs were synthesized. |
URI: | http://hdl.handle.net/11536/24312 http://dx.doi.org/10.1039/c4cc01227e |
ISSN: | 1359-7345 |
DOI: | 10.1039/c4cc01227e |
期刊: | CHEMICAL COMMUNICATIONS |
Volume: | 50 |
Issue: | 43 |
起始頁: | 5786 |
結束頁: | 5789 |
顯示於類別: | 期刊論文 |