標題: Synergistic hierarchical silicone-modified polysaccharide hybrid as a soft scaffold to control cell adhesion and proliferation
作者: Huang, Wei-Chen
Liu, Kun-Ho
Liu, Ta-Chung
Liu, Dean-Mo
Chen, San-Yuan
材料科學與工程學系
Department of Materials Science and Engineering
關鍵字: Amphiphilic copolymer;Cell adhesion;Interface manipulation;Mechanical stiffness;Topography
公開日期: 1-八月-2014
摘要: In this study, a new type of polydimethylsiloxane-modified chitosan (PMSC) amphiphilic hydrogel was developed as a soft substrate to explore cellular responses for dermal reconstruction. The hydrogel wettability, mechanical stiffness and topography were controllable through manipulation of the degree of esterification (DE) between hydrophobic polydimethylsiloxane (PDMS) and hydrophilic N,O-(carboxymethyl)-chitosan (NOCC). Based on microphase separation, the incorporation of PDMS into NOCC increased the stiffness of the hybrid through the formation of self-assembled aggregates, which also provided anchor sites for cell adhesion. As the DE exceeded 0.39, the size of the PDMS-rich aggregates changed from nanoscale to microscale. Subsequently, the hierarchical architecture resulted in an increase in the tensile modulus of the hybrid gel up to fourfold, which simultaneously provided mechano-topographic guidance and allowed the cells to completely spread to form spindle shapes instead of forming a spherical morphology, as on NOCC (DE = 0). The results revealed that the incorporation of hydrophobic PDMS not only impeded acidic damage resulting from NOCC but also acted as an adhesion modification agent to facilitate long-term cell adhesion and proliferation on the soft substrate. As proved by the promotion on long-term type-I collagen production, the PMSC hybrid with self-assembled mechano-topography offers great promise as an advanced scaffold material for use in healing applications. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.actbio.2014.04.025
http://hdl.handle.net/11536/24853
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2014.04.025
期刊: ACTA BIOMATERIALIA
Volume: 10
Issue: 8
起始頁: 3546
結束頁: 3556
顯示於類別:期刊論文


文件中的檔案:

  1. 000339459500017.pdf

若為 zip 檔案,請下載檔案解壓縮後,用瀏覽器開啟資料夾中的 index.html 瀏覽全文。