標題: | Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus |
作者: | Hsieh, Yin-Cheng Chiu, Hsi-Ho Huang, Yen-Chieh Fun, Hoong-Kun Lu, Chia-Yu Li, Yaw-Kuen Chen, Chun-Jung 交大名義發表 應用化學系 National Chiao Tung University Department of Applied Chemistry |
公開日期: | 1-九月-2014 |
摘要: | Glycosyltransferases (GTs), which are distributed widely in various organisms, including bacteria, fungi, plants and animals, play a role in synthesizing biological compounds. Glycosyltransferase-1 from Bacillus cereus (BcGT-1), which is capable of transferring glucose to small molecules such as kaempferol and quercetin, has been identified as a member of the family 1 glycosyltransferases which utilize uridine diphosphate glucose (UDP-glucose) as the sugar donor. BcGT-1 (molecular mass 45.5 kDa) has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction of BcGT-1 crystals to 2.10 angstrom resolution, the crystal belonged to space group P1, with unit-cell parameters a = 54.56, b = 84.81, c = 100.12 A, alpha = 78.36, beta = 84.66, gamma = 84.84 degrees. Preliminary analysis indicates the presence of four BcGT-1 molecules in the asymmetric unit with a solvent content of 50.27%. |
URI: | http://dx.doi.org/10.1107/S2053230X14014629 http://hdl.handle.net/11536/25200 |
ISSN: | 1744-3091 |
DOI: | 10.1107/S2053230X14014629 |
期刊: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS |
Volume: | 70 |
Issue: | |
起始頁: | 1228 |
結束頁: | 1231 |
顯示於類別: | 期刊論文 |