標題: A fluorescence turn-on probe for cysteine and homocysteine based on thiol-triggered benzothiazolidine ring formation
作者: Liu, Shi-Rong
Chang, Chao-Yiu
Wu, Shu-Pao
應用化學系
Department of Applied Chemistry
公開日期: 7-Nov-2014
摘要: We synthesized a new coumarin-based probe TP, containing a disulfide moiety, to detect biothiols in cells. A fluorescence turn-on response is induced by the thiol-disulfide exchange of the probe, with subsequent intramolecular benzothiazolidine ring formation giving rise to a fluorescent product. The probe exhibits an excellent selectivity for cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH) and other amino acids. The fluorescent probe also exhibits a highly sensitive fluorescence turn-on response to Cys and Hcy with detection limits of 0.8 mu M for Cys and 0.5 mu M for Hcy. In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the probe TP could be an efficient fluorescent detector for thiols in living cells. (C) 2014 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.aca.2014.08.024
http://hdl.handle.net/11536/25316
ISSN: 0003-2670
DOI: 10.1016/j.aca.2014.08.024
期刊: ANALYTICA CHIMICA ACTA
Volume: 849
Issue: 
起始頁: 64
結束頁: 69
Appears in Collections:Articles