標題: Development of CpG-Oligodeoxynucleotides for Effective Activation of Rabbit TLR9 Mediated Immune Responses
作者: Chuang, Tsung-Hsien
Lai, Chao-Yang
Tseng, Ping-Hui
Yuan, Chiun-Jye
Hsu, Li-Chung
生物科技學系
Department of Biological Science and Technology
公開日期: 30-Sep-2014
摘要: CpG-oligodeoxynucleotides (CpG-ODN) are potent immune stimuli being developed for use as adjuvants in different species. Toll-like receptor 9 (TLR9) is the cellular receptor for CpG-ODN in mammalian cells. The CpG-ODN with 18-24 deoxynucleotides that are in current use for human and mouse cells, however, have low activity with rabbit TLR9. Using a cell-based activation assay, we developed a type of CpG-ODN containing a GACGTT or AACGTT motif in 12 phosphorothioate-modified deoxynucleotides with potent stimulatory activity for rabbit TLR9. The developed CpG-ODN have higher activities than other developed CpG-ODN in eliciting antigen-nonspecific immune responses in rabbit splenocytes. When mixed with an NJ85 peptide derived from rabbit hemorrhagic disease virus, they had potent activities to boost an antigen-specific T cell activation and antibody production in rabbits. Compared to Freund\'s adjuvant, the developed CpG-ODN are capable of boosting a potent and less toxic antibody response. The results of this study suggest that both the choice of CpG-motif and its length are important factors for CpG-ODN to effectively activate rabbit TLR9 mediated immune responses.
URI: http://dx.doi.org/10.1371/journal.pone.0108808
http://hdl.handle.net/11536/25367
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0108808
期刊: PLOS ONE
Volume: 9
Issue: 9
結束頁: 
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