標題: | Using biofunctionalized nanoparticles to probe pathogenic bacteria |
作者: | Ho, KC Tsai, PJ Lin, YS Chen, YC 應用化學系 Department of Applied Chemistry |
公開日期: | 15-Dec-2004 |
摘要: | In this paper, we report a method for fabricating biofunctionalized nanoparticles by attaching human immunoglobulin (IgG) onto their surfaces through either electrostatic interactions or covalent binding. We found that these IgG-presenting nanoparticles can bind selectively to the cell walls of pathogens that contain IgG-binding sites based on the investigation of transmission electron microscopy images. Our results demonstrate that such Au-IgG nanoparticles may serve as useful nanoscale probes for exploring the interactions between IgG and pathogens. Furthermore, the IgG-presenting magnetic nanoparticles have been employed as effective affinity probes for selectively concentrating traces of target bacteria from sample solutions. The trapped bacteria were then characterized by using matrix-assisted laser desorption/ionization mass spectrometry. The lowest cell concentration we detected for both Staphylococcus saprophyticus and Staphylococcus aureus in aqueous sample solutions (0.5 mL) was similar to3 x 10(5) cfu/mL, while the detectable cell concentration for S. saprophyticus in a urine sample was similar to3 x 10(7) cfu/mL. |
URI: | http://dx.doi.org/10.1021/ac048688b http://hdl.handle.net/11536/25549 |
ISSN: | 0003-2700 |
DOI: | 10.1021/ac048688b |
期刊: | ANALYTICAL CHEMISTRY |
Volume: | 76 |
Issue: | 24 |
起始頁: | 7162 |
結束頁: | 7168 |
Appears in Collections: | Articles |
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