完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chen, Chao-Hsuan | en_US |
dc.contributor.author | Cuong, Nguyen-Van | en_US |
dc.contributor.author | Chen, Yung-Tsung | en_US |
dc.contributor.author | So, Regina Cheng | en_US |
dc.contributor.author | Liau, Ian | en_US |
dc.contributor.author | Hsieh, Ming-Fa | en_US |
dc.date.accessioned | 2014-12-08T15:38:05Z | - |
dc.date.available | 2014-12-08T15:38:05Z | - |
dc.date.issued | 2011-01-01 | en_US |
dc.identifier.issn | 1533-4880 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1166/jnn.2011.3102 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/26123 | - |
dc.description.abstract | The amphiphilic block copolymer methoxy-poly(ethylene glycol)-poly(epsilon-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce nano-sized micellar nanoparticles. The nanoparticles were loaded with anti-tumor drug, doxorubicin (DOX) and the size of the DOX-loaded nanoparticles were determined by dynamic light scattering (DLS) in aqueous solution to be from 197.4 to 230 nm. The nanoparticles subjected to co-culture with macrophage cells showed that these nanoparticles used as drug carrier are not recognized as foreign bodies. Overexpression of P-glycoprotein (P-gp) is an important factor in the development of multidrug resistance (MDR) in many cancer cells. In this study, Western blot and Rhodamine 123 were used to monitor the relative P-glycoprotein expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. The endocytosis of the DOX-loaded nanoparticles by breast cancer cells is more efficient observed under a confocal laser scanning microscopy (CLSM) and a flow cytometry in MCF7/ADR cells, compared to the diffusion of the free drug into the cytoplasm of cells. Based on these findings, we concluded that the nanoparticles made from mPEG-PCL-g-cellulose were effective in overcoming P-gp efflux in MDR breast cancer cells. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | Methoxy-Poly(ethylene glycol) | en_US |
dc.subject | Poly(epsilon-caprolactone) | en_US |
dc.subject | Multidrug Resistance | en_US |
dc.subject | P-Glycoprotein | en_US |
dc.subject | Human Breast Cancer Cells | en_US |
dc.title | Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Doxorubicin Nanoparticles of mPEG-PCL-Graft-Cellulose | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1166/jnn.2011.3102 | en_US |
dc.identifier.journal | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | en_US |
dc.citation.volume | 11 | en_US |
dc.citation.issue | 1 | en_US |
dc.citation.spage | 53 | en_US |
dc.citation.epage | 60 | en_US |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | 應用化學系分子科學碩博班 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.contributor.department | Institute of Molecular science | en_US |
dc.identifier.wosnumber | WOS:000286344400005 | - |
dc.citation.woscount | 13 | - |
顯示於類別: | 期刊論文 |