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dc.contributor.authorChen, Chao-Hsuanen_US
dc.contributor.authorCuong, Nguyen-Vanen_US
dc.contributor.authorChen, Yung-Tsungen_US
dc.contributor.authorSo, Regina Chengen_US
dc.contributor.authorLiau, Ianen_US
dc.contributor.authorHsieh, Ming-Faen_US
dc.date.accessioned2014-12-08T15:38:05Z-
dc.date.available2014-12-08T15:38:05Z-
dc.date.issued2011-01-01en_US
dc.identifier.issn1533-4880en_US
dc.identifier.urihttp://dx.doi.org/10.1166/jnn.2011.3102en_US
dc.identifier.urihttp://hdl.handle.net/11536/26123-
dc.description.abstractThe amphiphilic block copolymer methoxy-poly(ethylene glycol)-poly(epsilon-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce nano-sized micellar nanoparticles. The nanoparticles were loaded with anti-tumor drug, doxorubicin (DOX) and the size of the DOX-loaded nanoparticles were determined by dynamic light scattering (DLS) in aqueous solution to be from 197.4 to 230 nm. The nanoparticles subjected to co-culture with macrophage cells showed that these nanoparticles used as drug carrier are not recognized as foreign bodies. Overexpression of P-glycoprotein (P-gp) is an important factor in the development of multidrug resistance (MDR) in many cancer cells. In this study, Western blot and Rhodamine 123 were used to monitor the relative P-glycoprotein expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. The endocytosis of the DOX-loaded nanoparticles by breast cancer cells is more efficient observed under a confocal laser scanning microscopy (CLSM) and a flow cytometry in MCF7/ADR cells, compared to the diffusion of the free drug into the cytoplasm of cells. Based on these findings, we concluded that the nanoparticles made from mPEG-PCL-g-cellulose were effective in overcoming P-gp efflux in MDR breast cancer cells.en_US
dc.language.isoen_USen_US
dc.subjectMethoxy-Poly(ethylene glycol)en_US
dc.subjectPoly(epsilon-caprolactone)en_US
dc.subjectMultidrug Resistanceen_US
dc.subjectP-Glycoproteinen_US
dc.subjectHuman Breast Cancer Cellsen_US
dc.titleOvercoming Multidrug Resistance of Breast Cancer Cells by the Micellar Doxorubicin Nanoparticles of mPEG-PCL-Graft-Celluloseen_US
dc.typeArticleen_US
dc.identifier.doi10.1166/jnn.2011.3102en_US
dc.identifier.journalJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGYen_US
dc.citation.volume11en_US
dc.citation.issue1en_US
dc.citation.spage53en_US
dc.citation.epage60en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000286344400005-
dc.citation.woscount13-
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