Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Okuno, Masanari | en_US |
dc.contributor.author | Hamaguchi, Hiro-o | en_US |
dc.date.accessioned | 2014-12-08T15:38:17Z | - |
dc.date.available | 2014-12-08T15:38:17Z | - |
dc.date.issued | 2010-12-15 | en_US |
dc.identifier.issn | 0146-9592 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1364/OL.35.004096 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/26225 | - |
dc.description.abstract | We have developed a multifocus confocal Raman microspectroscopic system for the fast multimode vibrational imaging of living cells. It consists of an inverted microscope equipped with a microlens array, a pinhole array, a fiber bundle, and a multichannel Raman spectrometer. Forty-eight Raman spectra from 48 foci under the microscope are simultaneously obtained by using multifocus excitation and image-compression techniques. The multifocus confocal configuration suppresses the background generated from the cover glass and the cell culturing medium so that high-contrast images are obtainable with a short accumulation time. The system enables us to obtain multimode (10 different vibrational modes) vibrational images of living cells in tens of seconds with only 1 mW laser power at one focal point. This image acquisition time is more than 10 times faster than that in conventional single-focus Raman microspectroscopy. (C) 2010 Optical Society of America | en_US |
dc.language.iso | en_US | en_US |
dc.title | Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1364/OL.35.004096 | en_US |
dc.identifier.journal | OPTICS LETTERS | en_US |
dc.citation.volume | 35 | en_US |
dc.citation.issue | 24 | en_US |
dc.citation.spage | 4096 | en_US |
dc.citation.epage | 4098 | en_US |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | 應用化學系分子科學碩博班 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.contributor.department | Institute of Molecular science | en_US |
dc.identifier.wosnumber | WOS:000285387300004 | - |
dc.citation.woscount | 17 | - |
Appears in Collections: | Articles |
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