標題: | Growth rate regulation of lac operon expression in Escherichia coli is cyclic AMP dependent |
作者: | Kuo, JT Chang, YJ Tseng, CP 生物科技學系 Department of Biological Science and Technology |
關鍵字: | growth rate;lac operon;cyclic adenosine monophosphate;Escherichia coli |
公開日期: | 23-十月-2003 |
摘要: | In contrast to the ribosomal RNA gene expression increasing with growth rate, transcription of the lac operon is downregulated by cell growth rate. In continuous culture, growth rate regulation of lac promoter was independent of carbon substrate used and its location on the chromosome. Since the lac operon is activated by cyclic adenosine monophosphate (cAMP), which decreases with increasing cell growth rate, expression of plac-lacZ reporter fusion was analyzed in cya mutant under various growth conditions. The results demonstrated that expression of plac-lacZ in cya mutant was both lower and growth rate independent. In addition, ppGpp (guanosine tetraphosphate) was not involved in the mechanism of growth rate regulation of the lac promoter. Thus, the results of this study indicate that cAMP mediates the growth rate-dependent regulation of lac operon expression in Escherichia coli. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. |
URI: | http://dx.doi.org/10.1016/S0014-5793(03)01071-8 http://hdl.handle.net/11536/27450 |
ISSN: | 0014-5793 |
DOI: | 10.1016/S0014-5793(03)01071-8 |
期刊: | FEBS LETTERS |
Volume: | 553 |
Issue: | 3 |
起始頁: | 397 |
結束頁: | 402 |
顯示於類別: | 期刊論文 |