標題: Identification of genes induced in vivo during Klebsiella pneumoniae CG43 infection
作者: Lai, YC
Peng, HL
Chang, HY
生物科技學系
Department of Biological Science and Technology
公開日期: 1-Nov-2001
摘要: A novel in vivo expression technology (IVET) was performed to identify Klebsiella pneumoniae CG43 genes that are specifically expressed during infection of BALB/c mice. The IVET employed a UDP glucose pyrophosphorylase (galU) -deficient mutant of K. pneumoniae which is incapable of utilizing galactose and synthesizing capsular polysaccharide, as demonstrated by its low virulence to BALB/c mice and a white nonmucoid colony morphology on MacConkey-galactose agar. By using a functional galU gene as the reporter, an RT, promoter could render the galU mutant virulent while maintaining the white nonmucoid colony phenotype. A total of 20 distinct sequences were obtained through the in vivo selection. Five of them have been identified previously as virulence-associated genes in other pathogens, while another five with characterized functions are involved in regulation and transportation of nutrient uptake, biosynthesis of isoprenoids, and protein folding. No known functions have been attributed to the other 10 sequences. We have also demonstrated that 2 of the 20 IVE genes turn on under iron deprivation, whereas the expression of another five genes was found to be activated in the presence of paraquat, a superoxide generator.
URI: http://dx.doi.org/10.1128/IAI.69.11.7140-7145.2001
http://hdl.handle.net/11536/29266
ISSN: 0019-9567
DOI: 10.1128/IAI.69.11.7140-7145.2001
期刊: INFECTION AND IMMUNITY
Volume: 69
Issue: 11
起始頁: 7140
結束頁: 7145
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