標題: Rapid process for purification of an extracellular beta-xylosidase by aqueous two-phase extraction
作者: Pan, IH
Li, YK
應用化學系
Department of Applied Chemistry
關鍵字: aqueous two-phase extraction;enzymes;beta-xylosidase
公開日期: 15-Apr-2001
摘要: A rapid process for purification of an extracellular beta -xylosidase with high purity was developed. The manipulation involved the precipitation of protein from culture medium and the extraction of enzyme from the resuspended elude protein solution by an aqueous-two phase separation. A linear random copolymer, PE62, with 20% ethylene oxide and 80% propylene oxide was employed in both stages of the purification. The enzyme was precipitated effectively by using 10% (w/v) PE62 and 5% (w/v) Na2HPO4. The aqueous two-phase extraction was performed with PE62 (10%)-NaH2PO4 (15%) as phase-forming reagent. SDS-PAGE analysis revealed that the purified enzyme is near homogeneity. The yield is about 100% with a purification factor of 8.8-fold. The whole process can be completed within an hour without any column chromatography. (C) 2001 Elsevier Science B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/S0378-4347(00)00604-6
http://hdl.handle.net/11536/29706
ISSN: 0378-4347
DOI: 10.1016/S0378-4347(00)00604-6
期刊: JOURNAL OF CHROMATOGRAPHY B
Volume: 754
Issue: 1
起始頁: 179
結束頁: 184
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