標題: Nanoparticle-enhanced Magnetic Field Induces Apoptosis in Nematode
作者: Huang, G. S.
Yeh, L. K.
Chen, Y. C.
材料科學與工程學系奈米科技碩博班
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
關鍵字: apoptosis;nanoparticles;magnetic field;C. elegans
公開日期: 2008
摘要: To investigate molecular consequence of magnetic field to C. elegans, Fe3O4 nanoparticles were incorporated into the culture media of C. elegans and the nematodes were grown under magnetic field for 4 days. For the expermental group, we observed a 30 to 50 percents reduction in the moving speed. However, only nematode at L4 stage exhibited this difference. To explore molecular mechanisms affected by this treatment, real-time RT-PCR was performed using primers specific to amplify 120 genes. Differential expression for genes belong to apoptosis was observed. Biochemical assay for Caspase 3 activity was performed. The magnetic field-treated nematode exhibited high levels of Caspase 3 activity consistent with real-time PRC. Fluorescence microscopy showed that apoptotic activity was associated with the Fe3O4 treatment. For further validation, a ced-3 mutant nematode under enhanced magnetic field showed less effect for the movement than wild-type. The current study indicated that nanoparticle-enhanced magnetic field induced apoptosis in nematode.
URI: http://hdl.handle.net/11536/30965
ISBN: 978-1-4200-8504-4
期刊: NSTI NANOTECH 2008, VOL 2, TECHNICAL PROCEEDINGS
起始頁: 497
結束頁: 500
Appears in Collections:Conferences Paper