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dc.contributor.authorLin, Kuan-Yuen_US
dc.contributor.authorCheng, Chi-Pingen_US
dc.contributor.authorChang, Bill Chia-Hanen_US
dc.contributor.authorWang, Wei-Chien_US
dc.contributor.authorHuang, Ying-Wenen_US
dc.contributor.authorLee, Yun-Shienen_US
dc.contributor.authorHuang, Hsien-Daen_US
dc.contributor.authorHsu, Yau-Heiuen_US
dc.contributor.authorLin, Na-Shengen_US
dc.date.accessioned2014-12-08T15:48:35Z-
dc.date.available2014-12-08T15:48:35Z-
dc.date.issued2010-08-02en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0011928en_US
dc.identifier.urihttp://hdl.handle.net/11536/32314-
dc.description.abstractBackground: Satellite RNAs (satRNAs), virus parasites, are exclusively associated with plant virus infection and have attracted much interest over the last 3 decades. Upon virus infection, virus-specific small interfering RNAs (vsiRNAs) are produced by dicer-like (DCL) endoribonucleases for anti-viral defense. The composition of vsiRNAs has been studied extensively; however, studies of satRNA-derived siRNAs (satsiRNAs) or siRNA profiles after satRNA co-infection are limited. Here, we report on the small RNA profiles associated with infection with Bamboo mosaic virus (BaMV) and its two satellite RNAs (satBaMVs) in Nicotiana benthamiana and Arabidopsis thaliana. Methodology/Principal Findings: Leaves of N. benthamiana or A. thaliana inoculated with water, BaMV alone or coinoculated with interfering or noninterfering satBaMV were collected for RNA extraction, then large-scale Solexa sequencing. Up to about 20% of total siRNAs as BaMV-specific siRNAs were accumulated in highly susceptible N. benthamiana leaves inoculated with BaMV alone or co-inoculated with noninterfering satBaMV; however, only about 0.1% of vsiRNAs were produced in plants co-infected with interfering satBaMV. The abundant region of siRNA distribution along BaMV and satBaMV genomes differed by host but not by co-infection with satBaMV. Most of the BaMV and satBaMV siRNAs were 21 or 22 nt, of both (+) and (-) polarities; however, a higher proportion of 22-nt BaMV and satBaMV siRNAs were generated in N. benthamiana than in A. thaliana. Furthermore, the proportion of non-viral 24-nt siRNAs was greatly increased in N. benthamiana after virus infection. Conclusions/Significance: The overall composition of vsiRNAs and satsiRNAs in the infected plants reflect the combined action of virus, satRNA and different DCLs in host plants. Our findings suggest that the structure and/or sequence demands of various DCLs in different hosts may result in differential susceptibility to the same virus. DCL2 producing 24-nt siRNAs under biotic stresses may play a vital role in the antiviral mechanism in N. benthamiana.en_US
dc.language.isoen_USen_US
dc.titleGlobal Analyses of Small Interfering RNAs Derived from Bamboo mosaic virus and Its Associated Satellite RNAs in Different Plantsen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0011928en_US
dc.identifier.journalPLOS ONEen_US
dc.citation.volume5en_US
dc.citation.issue8en_US
dc.citation.epageen_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department生物資訊及系統生物研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitude of Bioinformatics and Systems Biologyen_US
dc.identifier.wosnumberWOS:000280520400014-
dc.citation.woscount11-
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