金奈米粒子對溶菌酶結構及活性影響

Abstract

我們從微觀的角度－奈米粒子與生物分子間的交互作用－去探討並觀察金奈米粒子對溶菌酶性質、活性的影響，並解釋金奈米粒子與溶菌酶彼此間的作用力。根據實驗結果發現，不同粒徑金奈米粒子使溶菌酶活性降低約10%，且粒徑越大活性相對降低越多。金奈米粒子雖然對溶菌酶酵素活有抑制的作用，但並不會對溶菌酶結構造成影響。從X光繞射數據所得到的結構分析，推測金奈米粒子與溶菌酶之間吸附作用力主要是以親疏水作用力互相吸附，並非具有專一性吸附在某些帶特定電荷的殘基附近。

The binding of protein to solid surfaces often induces changes in the structure and activity. Here we studied the activity, structural change and interaction of chicken egg white lysozyme on gold nanoparticles(GNPs) of different diameters. The activity of lysozyme adsorbed on gold nanoparticles with different size decrease about 10% to free lysozyme. Even though the gold nanoparticles decrease the activity of lysozyme, but it does not change the conformation of lysozyme. A comparative analysis of variation in X-ray diffraction (XRD) data revealed the conformational change and interaction with gold nanoparticles at atomic resolution, we proposed that the mainly interaction between gold nanoparticle and lysozyme is the adsorption of hydrophobic/hydrophilic interaction, but not adsorb around certain of specific charged residues.