生物官能基化奈米鑽石性質及其應用

Abstract

iamond with good photostability, easy surface-modification, and low cytotoxicity is a good nanomaterial for bio-applications. In the first part of this thesis, we develop a new technique which is combination of SAM and E-beam lithography to pattern nanodiamond on silicon base substrate. The technique which we demonstrated here is suitable for applications of bio-sensing chips and single bio-molecule patterning and detection. For the further works, we expect to develop for bio-chip base on bio-functionalozed nanodimond arrays. In the second part of this thesis, we couple nanodiamond with gold nanoparticles of different sizes by using two complementary DNA sequences. After the gold nanoparticles were hybridized on the nanodiamonds, we observed enhancement of the photoluminescence (PL) signals originated from nanodiamond’s nitrogen-vacancy center. The enhancement was attributed to the plasmon field created by the gold nanoparticles. The shape of the enhanced PL spectra was also affected by the sizes of the attached nanoparticles due to their different resonant plasma frequency. The signal enhancement can be used as an indexing tool for bio-sensing purposes.

Diamond with good photostability, easy surface-modification, and low cytotoxicity is a good nanomaterial for bio-applications. In the first part of this thesis, we develop a new technique which is combination of SAM and E-beam lithography to pattern nanodiamond on silicon base substrate. The technique which we demonstrated here is suitable for applications of bio-sensing chips and single bio-molecule patterning and detection. For the further works, we expect to develop for bio-chip base on bio-functionalozed nanodimond arrays. In the second part of this thesis, we couple nanodiamond with gold nanoparticles of different sizes by using two complementary DNA sequences. After the gold nanoparticles were hybridized on the nanodiamonds, we observed enhancement of the photoluminescence (PL) signals originated from nanodiamond’s nitrogen-vacancy center. The enhancement was attributed to the plasmon field created by the gold nanoparticles. The shape of the enhanced PL spectra was also affected by the sizes of the attached nanoparticles due to their different resonant plasma frequency. The signal enhancement can be used as an indexing tool for bio-sensing purposes.