奈米線場效電晶體生物感測器於腸病毒71型之高靈敏度、無標記且即時檢測

Abstract

對世界各地的幼童而言腸病毒七十一型 (Enterovirus 71, EV71)是一種重要的致病原且比其他非小兒麻痺腸病毒 (non-polio enterovirus)具有高致病率及致死率，其感染屬於神經性症狀，且平均會在三天內惡化。傳統的臨床確認檢驗方式需要先病毒培養再進行病毒分離 (virus isolation)和藉由反轉錄聚合酶鏈式反應(RT-PCR)，這些過程耗時、昂貴且無法達到立即診斷EV71。在文獻中，多晶矽奈米線場效電晶 (polysilicon nanowire field-effect transistor, poly SiNW- FET)可被製成且具有高靈敏度、無標誌且立即偵測腸病毒七十一型的置能轉換器 (transducer)。對特定EV71的DNA序列有專一性的單股DNA序列先被固定在多晶矽奈米線場效電晶體表面，用來偵測EV71的多晶矽奈米線場效電晶體具有高靈敏度，且能對EV71產生反應，並且在有無交互作用的離子分子下仍是穩定的，最低可偵測到aM (attomolar, aM,10-18M)範圍。此結果表示多晶矽奈米線場線電晶體具有靈敏、無標誌且可立即偵測的淺能，此特性可發展成生物感測系統用來偵測EV71的感染，以便早期發現早期治療，

Enterovirus 71 (EV71) is an important pathogen that cause higher morbidity and mortality in children around the world than those of other non-polio enteroviruses. Its infection is neurotropic and followed by rapid deterioration within average 3 days. The conventional clinical methods for EV71 identification require virus isolation from cell culture and DNA amplification by reverse transcription polymerase chain reactions (RT-PCR), which is time-consuming, expensive and cannot meet the urgent need for the diagnosis of EV71. In this report, polysilicon nanowire field-effect transistor (poly SiNW- FET) was fabricated and function as a transducer for ultrasensitive, label-free and real-time detection of EV71. Specific single-strand DNA sequences that the unique DNA sequence of EV71 were first immobilized on poly SiNW-FET. The fabricated poly SiNW-FET based EV71 biosensor exhibit a high sensitive and specific in respond to EV71. The functionalized poly SiNW-FET was stable in the presence of non-interacting ion molecules and was able to detect EV71 RNA at the aM range. The results of this study suggest that the poly SiNW-FET has a potential to be useful developed to a real-time, sensitive and label-free detection. The characteristics make it a potential biosensing system for early recognition and give treatment for EV71 infection.