酪胺酸酶電化學感測器檢測內分泌干擾物及酚類污染物

Abstract

酚類化合物是由合成後的化學物質常作為人工內分泌物(內分泌干擾物)或是環境汙染物。內分泌干擾物的作用主要是透過模仿正常荷爾蒙的功能來影響人類健康。酚類污染物通常是具有細胞毒性的，且廣泛的存在於廢水、民生用品、家庭用品等。這些存在於環境當中的汙染物通常是極少量且不易用廉價的儀器來發現。因此有此必要發展一種靈敏且操作容易的感測器來偵測這些汙染物。 此篇論文，在發展以酪胺酸酶(tyrosinase)基礎的生物感測器，藉由將酪胺酸酶(tyrosinase) 固定化在碳纖維紙上來偵測酚類化合物。對於內分泌干擾物或酚類污染物之偵測呈現出具有高靈敏性。對於Catechol、phenol、bisphenol A、3-amiophenol ，其生物感測器之電化學應答各別為3.74, 1.49, 1.09 and 0.78 A M-1。對於偵測Catechol、phenol、bisphenol A、3-amiophenol的線性範圍分別為；0.01 ~ 15 μM 、 0.01 ~ 15 μM 、 0.01 ~ 1 μM 、 0.02 ~ 1 μM。偵測極限分別為 2、5、5、12 nM。然而此製備的酪 酸酶生物感測器具有良好的重覆使用性，相對標準差為5.02%。

Phenolic compounds are synthetic chemical components that usually act as artificial endocrines (called endocrine disrupting compounds) or environmental pollutants. Endocrine disrupting compounds (EDCs) affect human health by mimicking the function of normal hormones. Phenolic pollutants are usually cyto-toxic, and broadly exist in waste water, people's livelihood articles and household products. The amount of these pollutants in the environments is usually tiny and can not be found readily without expensive instruments. Thus, it is necessary to develop a sensitive and easily operated biosensor for the detection of these pollutants. In this thesis, a tyrosinase-based biosensor was developed for the detection of phenolic compounds by immobilizing tyrosinase on the carbon fiber paper (CFP) electrode. The developed tyrosinase biosensor exhibited high sensitivity for the detection of EDCs and phenolic compounds. The electrochemical response of biosensor to catechol, phenol, bisphenol A and 3-aminophenol was 3.74, 1.49, 1.09 and 0.78 A M-1, respectively. The linear range of detection for catechol, phenol, bisphenol A and 3-aminophenol was 0.01-15 μM , 0.01-15 μM , 0.01-1 μM and 0.02 -1 μM, respectively. The detection limit for catechol, phenol, bisphenol A and 3-aminophenol was 2, 5, 5, and 12 nM, respectively. Moreover, the fabricated tyrosinase biosensor exhibits a good repeatability with a relative standard deviation of 5.02%.