兆赫技術應用於豬皮燒傷深度的檢驗及血紅素光譜量測

Abstract

兆赫時域光譜技術可應用於兆赫波段光譜的研究。此研究工作利用兆赫時域光譜技術獲得正常與燒燙傷豬皮的折射係數與吸收係數，在0.6兆赫的折射係數分別為1.68 ± 0.01與1.76 ± 0.02，吸收係數則為12.4cm-1與10.8cm-1。所有的豬皮樣品均經由丙酮溶液去除水分以減少水對兆赫波訊號的吸收。這個技術也用來觀察到燒傷豬皮雙折射特性減弱的情形。此外，兆赫時域光譜技術也用來獲得粉末狀以及薄膜狀血紅素在兆赫波段的光譜資訊，血紅素在0.2到1.2兆赫的範圍有寬頻的吸收，用這個方法得到粉末狀及薄膜狀血紅素在0.1兆赫的折射係數分別為1.86 ± 0.01與1.61 ± 0.05，吸收係數則為25cm-1與 30cm-1。我們進一步應用兆赫光譜技術來作樣品反射式訊號的量測，可以觀察到用來模擬部分燒傷的皮膚的由一層燒燙傷以及一層正常的豬皮樣品堆疊成的結構中的界面，經由這個模擬結構表面以及內部界面和背面反射的訊號波形都可以正確清楚地判別，所得到反射的波形以及表面和界面間的飛行時間和個別量測正常及燒燙傷樣品的結果吻合。經由這些初步的實驗結果應用到真實燒燙傷深度的判斷將是有可能的。

THz time domain spectroscopy (THz-TDS) is for spectroscopy studies in THz ranges. In this work, THz-TDS is applied to characterize refractive indices and absorption coefficients of normal and burned porcine skin of 1.68 ± 0.01 and 1.76 ± 0.02, 12.4cm-1 and 10.8cm-1 at 0.6THz, respectively. All of the skin samples are dehydrated with Acetone to reduce the water absorption in THz ranges. Birefringence reduction of burned porcine skin has also been observed by this technology. In addition, the spectroscopic information of powder and thin-film porcine hemoglobin in THz range is acquired. Hemoglobin has broadband absorption from 0.2THz to 1.2THz. The measured refractive indices and absorption coefficients of powder and thin-film hemoglobin are 1.86 ± 0.01 and 1.61 ± 0.05, 25cm-1 and 30cm-1 at 1THz. Further reflective type measurement can be implemented by THz-TDS. Interface information is obtained from the measured THz waveform reflected from stacked burned and normal porcine skins for simulating partial burned skin. Reflective wave from surface, interface between burned and unburned, back surface of unburned porcine had been correctively and clearly observed. Waveform and time of flight matched well with the original reflected waveform of normal and burned porcine skin. Clinical burned depth detection will be possible from these initial experiments.