奈米材料添加對纖維母細胞成長之影響

Abstract

本研究主要是探討不同的材料來進行細胞培養對NIH3T3纖維母細胞貼附及增殖的影響，利用掃描式電子顯微鏡分析及觀察細胞的形態。研究結果顯示，纖維母細胞可在1~2天內附著在基材及所有鍍層表面，而表面粗糙度會影響細胞在基材上貼附情況。在光滑表面 (培養皿及鈦基材)，細胞從初期漸漸貼附於基材；在粗糙表面(奈米碳管/鈦片基材)鍍層上，細胞與細胞之間有細長的假足絲相連接至基材上。另外，纖維母細胞經培養觀察其貼附情形，奈米碳管/鈦片基材鍍層比鈦片基材、培養皿佳。從SEM觀察可發現，奈米碳管/鈦片基材鍍層之細胞貼附生長攤平的能力較培養皿及鈦片基材良好且快速，顯示奈米碳管鍍層於鈦合金上具有優良的生物相容性。

This investigation is focused on cultures with NIH3T3 fibroblasts, in order to observe the effect of different materials on adherence, growth and proliferation. A scanning electron microscope was used to characterize cell morphology. The fibroblasts were able to attach on all substrates and coatings after 1 to 2 days. On smooth surface (flask and Ti substrates), cells became gradually attached on substrates at early phases; on rough-surface materials (CNTs/Ti ), thin and long filopodia between the cells were connected to the substrate. Surface roughness has significant effect on the cell morphology and cell adhesion number. Additionally , the fibroblasts under SEM showed better and faster attachment and greater extension on CNTs/Ti coatings than flask and Ti alloy. This indicates that the carbon nanotube composite coating on Ti alloy has remarkable biocompatibility.