矽氧烷混成2-羥基乙基丙烯酸甲酯水膠設計: 分子結構特性、生物相容性與性質探討

Abstract

Silicone hydrogel has been received enormous interest in recent years because of its superb oxygen permeability. Among them, hydrophilic agents were introduced for hydrophilic modification. However, the ratio of the hydrophilic agents and the diluents had to be strictly controlled in order to inhibit phase separation and maintain the transparency. In this work, on the basis of traditional hydrogel, poly-HEMA, we successfully developed a novel hydrogel, poly-HEMA-silicone hydrogel, which can be cured by UV to form transparent hydrogel. To investigate the chemical structure and the proportion of siloxane structures, the hydrogel were characterized by FT-IR, 1H-, 29Si-nuclear magnetic resonance. The morphology was characterized by SEM and TEM, and the appearance of silica nano-particles was attributed to the result of the fully condensed silane structure. In addition, the distribution of hydrophobic silane domains was related to the physical properties of the hydrogel. Based on the result of the swelling test, we inferred that the hydrophilicity of the hydrogel was retained by the silanol groups on the outer silane domains, but the result of the protein test implied that the proteins preferentially deposited inside the loosely bounded hydrophobic silane domains without hydrophilic groups. Besides, the effect of the siloxane content on drug delivery behavior of HA hydrogel was also investigated. The second part of this work was to improve the silane content by incorporating PDMS. We successfully grafted HEMA and functionalized silane monomers at PDMS terminals and obviated phase separation problems, thus, the transmittance of the PDMS-containing hydrogel (HAS) was above 90% at visible range. FT-IR, 1H-, 29Si- NMR were also applied to investigate the siloxane structures. The morphology was also investigated by SEM and TEM. The hydrogels with different PDMS content showed different pore size distribution, which was related to the swelling ratio and the mechanical properties, and the relationships were also studied. Moreover, the observation on protein adsorption test revealed the incorporation of PDMS influencing the mean distance between polymer chains. Additionally, the cytotoxicity test against BCE cell line was applied on both of the hydrogels (HA and HSA), and the results supported their outstanding biocompatibility.

Silicone hydrogel has been received enormous interest in recent years because of its superb oxygen permeability. Among them, hydrophilic agents were introduced for hydrophilic modification. However, the ratio of the hydrophilic agents and the diluents had to be strictly controlled in order to inhibit phase separation and maintain the transparency. In this work, on the basis of traditional hydrogel, poly-HEMA, we successfully developed a novel hydrogel, poly-HEMA-silicone hydrogel, which can be cured by UV to form transparent hydrogel. To investigate the chemical structure and the proportion of siloxane structures, the hydrogel were characterized by FT-IR, 1H-, 29Si-nuclear magnetic resonance. The morphology was characterized by SEM and TEM, and the appearance of silica nano-particles was attributed to the result of the fully condensed silane structure. In addition, the distribution of hydrophobic silane domains was related to the physical properties of the hydrogel. Based on the result of the swelling test, we inferred that the hydrophilicity of the hydrogel was retained by the silanol groups on the outer silane domains, but the result of the protein test implied that the proteins preferentially deposited inside the loosely bounded hydrophobic silane domains without hydrophilic groups. Besides, the effect of the siloxane content on drug delivery behavior of HA hydrogel was also investigated. The second part of this work was to improve the silane content by incorporating PDMS. We successfully grafted HEMA and functionalized silane monomers at PDMS terminals and obviated phase separation problems, thus, the transmittance of the PDMS-containing hydrogel (HAS) was above 90% at visible range. FT-IR, 1H-, 29Si- NMR were also applied to investigate the siloxane structures. The morphology was also investigated by SEM and TEM. The hydrogels with different PDMS content showed different pore size distribution, which was related to the swelling ratio and the mechanical properties, and the relationships were also studied. Moreover, the observation on protein adsorption test revealed the incorporation of PDMS influencing the mean distance between polymer chains. Additionally, the cytotoxicity test against BCE cell line was applied on both of the hydrogels (HA and HSA), and the results supported their outstanding biocompatibility.